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We narrowed to 1,013 results for: CAL

Showing: 761 - 780 of 1013 results
  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038... Expression specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https...
  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons...in a genome. But there is a type of mobile DNA, called a transposon, that’s a bit hyperactive and likes...
  3. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...understanding of how protein folding works and creating biological tools not found in nature. Dr. Baker’s team was...was not based on any existing protein structures, called Top7. Figure 1: Top7 receives the Nobel ...
  4. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...of an “electricity aggregation program” for all local households and businesses. Electricity aggregation... energy expenditure here at Addgene. These are critical systems that cannot be removed or substantially...
  5. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind them you care!#thanks #...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...
  6. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...preparations of several variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now...preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so...
  7. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse...
  8. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...with most reagents sent, and the most recent full calendar year When the website is launched, it will show...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...
  9. Editor's Choice, October 2016

    Type
    Blog Post
    ...finishing up their time in the PhD Program in Biological and Biomedical Sciences at Harvard University. Ben and...
  10. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...E. coli, the Chong Zhang lab developed a genome-scale CRISPR inhibition pooled library (~60,000 gRNAs)...
  11. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... Support Specialists here at Addgene. If you’ve called our customer service line or sent an email to help...plasmids, store your plasmids or viral items, technical support, and more. ...
  12. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...
  13. CRISPR Library Amplification

    Type
    Protocol
    ...the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later...libraries up to 200,000 individual plasmids and can be scaled according to the actual number. Required Quality...selection). This recombination, at a low rate, is not typically a problem as these sequences are not efficiently...200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip...cells) Less library DNA can be used but this dramatically increases the chances of individual plasmids...Cell Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and ...normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare...
Showing: 761 - 780 of 1013 results