We narrowed to 1,081 results for: ANT

...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...recombinase. The high concentration of FLEx vectors guarantees that most cells are transfected with all labeling...versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible systems ...

...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...Easi-CRISPR for more details. References and resources Want to know more? Check out the additional resources...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

... monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid...CGE-LIF) for detection and can provide data on the quantity of various topologies and multimers present within...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

...d microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired...other homology-directed repair experiments, you’ll want the guide cut site to be as close as possible to...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

...your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

...For example, in bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids...is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells...2021). Target site preference and host range are important to consider when selecting a transposon for a ... Sleeping Beauty and piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ...

...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...acquired all fluorophores throughout the sample are constantly exposed to light, not just those in the focal...for this to happen.Using red light has a second advantage: red light penetrates deeper into biological tissue...-time monitoring of neuronal activity across substantial 3D volumes. In addition to 2-photon microscopy...

...2!) decades that it's hard to summarize, but we wanted to mark the occasion by trying.  When we started...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...Scientific Writing" on Sunday, from 12 pm - 2 pm. If you want to learn about, and practice, principles of clear...

...approximate, two-dimensional map. The Baker Lab wanted to create a new fold that is not present in nature...design model at atomic resolution” (Kuhlman and Dantas et al., 2003). That made it a powerful proof of...Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L....

... my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately...2022   Congratulations to all our #DeckTheLab contestants!           View this post on Instagram                     ... labs with everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance...

...depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual reports...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...metrics!  About the Author Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal precision...

...colony to DNA extraction and quantification so that you can focus on what you want to do with your plasmid ... grades of DNA  Read about the various ways to quantify DNA Resources on Addgene.org Learn about our...

...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

...into next year we’d like to know what topics you want to learn more about. Our post on “Making Your Own...Finally, we are always looking for guest bloggers who want to share their research with the community, so email...Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids...

...fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While...others may be less obvious — but every change is meant to make your experience on our website better so...

...programmable DNA-binding domains" publication.  If you want to see this new feature in action, click here to...plasmid of interest, which may help you identify relevant protocols and experimental data Find other related... an article that used a plasmid from Addgene, we want to hear about it so we can link to it in the "Articles...

...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...