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We narrowed to 957 results for: Cre

Showing: 761 - 780 of 957 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  3. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...the presence of your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or ...non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform called DETECTR. How does...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    ... you when you need it. The opportunity to be creative and think critically. One of the very first times...defined as sitting down at a computer and just creating, which for many scientists is the most enjoyable...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...gene disruption in Mycobacterium abscessus, an increasingly common human pathogen notorious for its high...adhesion to a PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes...

  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service...GCaMP6. It could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of survival. This...antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies, and investigate more...

  10. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    ...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

  12. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  13. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...
Showing: 761 - 780 of 957 results