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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 967 results for: Cre

Showing: 761 - 780 of 967 results

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and...have a kit!). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...learning from the careers of others were key to creating my career path. Luckily, in grad school much of...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...students, postdocs, and other working scientists, creating a presentation for 8th graders was a little intimidating...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...gRNA.  While this methodology has proven to be incredibly valuable to scientists, there are some potential...Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...researcher experiences and that it often provides incredibly valuable insight into one’s work. As a community...information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of survival. This...antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies, and investigate more...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...gene disruption in Mycobacterium abscessus, an increasingly common human pathogen notorious for its high...adhesion to a PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service...GCaMP6. It could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...the presence of your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or ...non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform called DETECTR. How does...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    ... you when you need it. The opportunity to be creative and think critically. One of the very first times...defined as sitting down at a computer and just creating, which for many scientists is the most enjoyable...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    ...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...
Showing: 761 - 780 of 967 results