We narrowed to 1,031 results for: ELL

... colleagues. When you attend a well-run meeting, note what worked well and ask the meeting leader for ...annoying interruptions to our “real” work. However, a well-run meeting can have quite the opposite effect. .... We’ve provided some tips above for what works well at Addgene, but meetings are never one-size-fits...

...expected to make sure the work gets done and done well. Involving your team effectively is now your new... in mind, you won’t be able to communicate these well to the task assignee and this is a recipe for bad...delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities, good ...Delegating" in the Harvard Business Review. Another excellent blog on delegation tips and practices can be found...

... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent... Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role..., Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions...

... be wondering how this all fits in with science. Well, I’ll give you an example. At my previous company... goes into software product management? I always tell people the best part of my job is getting to talk...communicate with customers, software developers, as well as team members in automation, sales, marketing,...help you be a valuable asset to the scientists as well as the developers. Lastly, you’re going to need ...

...idea from academia to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing...aligned in vision and outcomes. The team must work well together, have complementary skill sets, and “talk...were free and free to repair.” If the team works well, the idea is solid, and “the pitch is packaged to...

...you correct any language- and spelling mistakes. A manuscript that is well written looks more professional...submission, missing files or a manuscript full of spelling errors will not have a great chance of getting...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ... our most popular lentiviral and AAV vectors, as well as developing and sharing supportive educational...stories we can, and to hear the stories others have to tell.  "Also thanks for being Addgene. You guys rock....

...enable scientific reproducibility. This workshop was well received, and then when the team started brainstorming...widespread issue of irreproducibility. Use your network well to seek out valuable and impactful opportunities...course! Thanks to individuals in the group who were well connected, this diverse and international team assembled...website How-to-guide for Reproducibility workshops Levelling Up: Reproducibility for Everyone Addgene: a global...

...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

...read a 100-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might...obtain?” If the answer is yes, you will need to spell out that connection for your audience. I ask myself...needed. The trick is to communicate your key points well enough that the audience can take that knowledge...

... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...generated against a linear antigen tend to work very well for applications that denature a protein to its ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody...References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T,...

... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...would result in a functional channelrhodopsin, as well as predict the channelrhodopsin’s photocurrent strength...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

...practical solutions.  My PhD prepared me remarkably well for this task. Good scientists learn how to communicate...to say “I’m going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different...different types of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create...It is never really done. But if you implement it well, here’s what can happen: More effective meetings...

...transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above)....integrates our database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for... be found here. Finally, you will want to have a well-organized database of expected sequences, either...some tips on how to get better sequencing reads? Tell us your favorite sequencing tricks in the comments...

... Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally, controls...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...

...colony.  You should genotype all of your F1 mice, as well as progeny from future generations. 4. Continue ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...on separate chromosomes in future generations as well, a concept known as linkage. This hinders the likelihood...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

...communication, and policy. In this post, I hope to tell you a little more about how I ended up at pgEd, ...starting conversations about both the benefits as well as the personal, social and ethical implications...broaden my training beyond the biological sciences, as well as to acquire leadership and communication experience...scientific, educational or industry conferences, as well as schools, community events, churches or mosques...

...answering help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that...company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data on the catalog...their products from you, unless they’re knowingly selling a bad product (in which case, you should probably... some time. If the customer service rep does not tell you upfront how long it will take to get an answer...

...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...tag and small molecule. It does work in mammalian cells, mice, and even yeast, though (we have a kit!). ...and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350...Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

... thought about management skills before being propelled into the position of manager. I barely had any...check myself sometimes to make sure I am delegating well, but it does get easier over time! Communicate often...to recognize someone’s hard work and reward a job well done. Your report will (needs to) feel valued. Talk...should help you work through your situation, not tell you what to do. Unfortunately this is not always...