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  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...mutations are essential data points for investigators looking to definitively link genotype and phenotype. It... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...cytometry particularly well to complex experiments looking at a variety of different targets. If you are planning...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...

  8. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...efficiency is so high that some applications may permit working with edited “pools” of cells, eliminating the time-consuming...telomere length. Telomere trimming can be assayed by looking for the production of ECT-DNA (extra-chromosomal...

  11. New Coomassie Protocol Video

    Type
    Blog Post
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  15. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...
Showing: 761 - 780 of 926 results