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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,018 results for: LEA

Showing: 761 - 780 of 1018 results

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  3. How Addgene Creates Content

    Type
    Blog Post
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  7. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  9. To Each HIS Own

    Type
    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  11. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  12. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...
Showing: 761 - 780 of 1018 results