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  1. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  2. Plasmids 101: Yeast Vectors

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    Blog Post
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  3. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  4. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  6. To Each HIS Own

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    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  7. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  10. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

  13. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...

  14. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...

  15. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post
    ...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...

  17. Plasmids 101: Modular Cloning

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    Blog Post
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

  19. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...

  20. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...
Showing: 761 - 780 of 1011 results