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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,028 results for: LEA

Showing: 761 - 780 of 1028 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  5. How Addgene Creates Content

    Type
    Blog Post
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  8. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...critical roles in nerve and muscle function, hormone release, and cell signaling throughout the body. Because...Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR). ...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  11. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  15. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  20. To Each HIS Own

    Type
    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...
Showing: 761 - 780 of 1028 results