We narrowed to 809 results for: Mak

1. Cpf1: A New Tool for CRISPR Genome Editing

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   ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

2. Your Lentiviral Plasmid FAQs Answered

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   ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

3. CRISPR-mediated Plant Base Editors

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   ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

4. Analyzing CRISPR Editing Results with ICE from Synthego

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   ...engineering has revolutionized the gene editing field by making experimental workflows considerably easier, faster...

5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...

6. Plasmids 101: Gateway Cloning

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   ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

7. To Codon Optimize or Not: That is the Question

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   ...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based on...

8. Plasmids 101: Plasmid Incompatibility

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   ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

9. Plasmids 101: Broad Host Range Plasmids

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   ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

10. Bioinformatics at Addgene

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    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

11. Transferable Skills Guide: Creativity

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    ...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

12. Antibodies 101: Immunoprecipitation

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    ... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

13. Antibodies 101: Reading a Flow Plot

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    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

14. Viral Vectors 101: Pseudotyping

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    ...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

15. Mesothelioma - Causes, Symptoms, and Treatment

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    ... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma...

16. 5 Reasons to Use Reddit for Science Communication

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    ...until 5 pm). The ability to see the questions first makes Reddit a great platform for those who aren’t so ...

17. CRISPR Activation: A Practical Guide

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    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

18. Tips for CRISPR Gene Editing in Mice

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    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

19. The Challenges of Cell Culture

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    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

20. Getting the Most from Your Lentiviral Transduction

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    ...physiology of different cell types, however, can make this assumption incorrect. One of the earliest steps...