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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 939 results for: OMP

Showing: 761 - 780 of 939 results

  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...

  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  5. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...

  14. Viral Production at Addgene

    Type
    Blog Post
    ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...

  17. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field with...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  19. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...
Showing: 761 - 780 of 939 results