We narrowed to 1,017 results for: PLE;

...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...a potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

...with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with using ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ...chromosomes essentially are messed up chromosomes with multiple inversions. They are unable to participate in ... dominant phenotypes visible on the flies. For example, second chromosome balancers often have the curly...

.... For more mechanistic details on HDR and NHEJ, please see the linked blog posts. Compared to its counterparts...strategy looks very similar to HDR, but it is completed using much shorter sequence homologies. Abbreviated...Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621-633. PMID: ...

.... Here is an example of a plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...

...ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting gRNA, to your cells of interest...CRISPR experiment is the generation of the RNP complex.  While one option is to purchase the Cas9 protein...bread wheat using CRISPR/Cas9 ribonucleoprotein complexes." Nature communications 8 (2017): 14261. PubMed...

.... Now GFP and its fluorescent derivatives are a staple in the lab. GFP is used in research across a vast...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...exist with only slight variations separating them.  Please note that many fluorescent proteins found on the...

...granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for...

...of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B. Read on to learn... the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced version of PHP.B... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many.... “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit ...

...GCN4 binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag ..., Alitheen NBM, Hamid M (2012) scFv Antibody: Principles and Clinical Application. Clinical and Developmental....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute...

...system frequently over the past fifteen years. Some examples from their lab include using AAV to introduce ... alleles of a gene, or which require modifying multiple genes simultaneously in the same cell, are more...by using a targeted nuclease. Asuri et al, for example, used zinc fingers to increase the efficiency of...

...pluripotent stem cells (iPSCs). The CRISPR/Cas9 com­plex is very efficient at introducing double stranded...the PAM as possible (see below to find out why). Please check our recent studies for details (Paquet, Kwart... systems.     References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...

...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different...and modifying capsid and genomic structures. For example, rAAV pseudotypes mix the genome of one AAV serotype... AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...

...allows for targeted delivery of Tet1 alone, or coupled with DNA oxidation and repair factors that allow...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

... viral DNA sequences and expression profile at multiple steps from plasmid amplification in bacteria to... set of injections into your animal model. For example, in a recent study by the Wickersham lab (Lavin...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...