We narrowed to 992 results for: Ran

1. Intro to Our Summer SciComm Series!

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   ...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

2. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ... discipline and reduces the dissemination of unwarranted claims or unacceptable interpretations. A good...studying plant development and abiotic stress tolerance, I have been peer-reviewing journal submissions...

3. Science Careers: Unruly Interests Feed Many Paths

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   ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...

4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van Haren ...fluorescent proteins (FP) with improved tissue penetrance and live cell imaging capabilities. Upon developing...

5. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   ...teaching community through the TRAINED initiative (Translating Research on Arabidopsis Into a Network of Educational...Center for Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your...

6. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...Sabrina Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized...contributed by Jennifer Tsang Figure 4: Double stranded DNA bound by tTALE-FP with the fluorescent protein...

7. Antibodies 101: Epitope Tags

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   ...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

8. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

9. Identifying Sequence Elements with SnapGene's Feature Database

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   ... for control elements such as promoters and transcription terminators, the boundaries are less obvious...to newly sequenced strains through our mismatch tolerant detection algorithm and thereby speed up the annotation...

10. Fluorescent Biosensors for Measuring Autophagic Flux

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    ...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

11. Plasmid Cloning by PCR

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    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

12. Addgene's Top 10 Blog Posts of 2015

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    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

13. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...play a role in many cell processes involving cell membrane dynamics. When they learned there was a disease...desire to understand the early events in the transformation of healthy cells into melanoma cells. They’...

14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...can generate a fluorescent protein fusion, transcriptional reporter, and loss-of-function allele in just...markers. Bob Goldstein’s lab previously used a transgene selection strategy for their C. elegans/CRISPR...

15. Visualizing Genomic Loci with CRISPR-Sirius

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    ...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes...

16. Addgene Depositors Get More Citations

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    ...on a range of topics including biotech and research tools, computing performance, and randomized control...

17. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    ... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...and do a quick break to chat. This can be done randomly or manually assigning which participants to group...

18. Networking Follow-up: I Met a Person. Now What?

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    ...enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

19. Hot Plasmids - January 2023

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    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

20. 5 Great Apps for Lab Life

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    ...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS...