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Showing: 761 - 780 of 1077 results
  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post
    ... using Nanoblades? Plasmids are available from Addgene. Genome editing primary cells with Nanoblades Primary...PMCID: PMC 6318322. Additional resources on the Addgene blog Learn about RNP delivery Learn about CRISPR...Browse our CRISPR 101 blog post Resources on Addgene.org Check out our comprehensive CRISPR guide Find...
  2. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...an extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...
  3. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...
  4. Technical Design of a Western Blot

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    Blog Post
    ...Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western Blot Protocol Video...Looking for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...Resources and references More resources on the Addgene blog Antibodies 101: The Basics of Western BlottingAntibodies... Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes...
  5. Antibodies 101: Flow Cytometry

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    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...
  6. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...
  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...
  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...
  9. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez ...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Validated...
  10. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...
  11. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references 1, 2....PMCID: PMC5609467. Additional Resources on the Addgene Blog Using virus in your research - a primer for...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...
  12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...
  13. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...
  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...
Showing: 761 - 780 of 1077 results