AAVs in Retinal Gene Therapy
Type
Blog Post
...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...
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MXS Chaining
TypeBlog Post...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...red lines), which maintains structural epitopes (orange region), but it can block epitopes hidden within... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...their science, meaning that they will heavily evangelize for the mode of communication they connect best... -
An Introduction to Adenovirus
TypeBlog Post... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs... -
Getting to Know Expansion Microscopy
TypeBlog Post...e73775. https://doi.org/10.7554/elife.73775 Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... you’re more of a leader than you thought! When change happens at your organization, when your boss needs... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol... Nonetheless, we recommend performing a buffer exchange before using the purified AAV in vivo . The iodixanol... Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps could be completed...Pool clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188 Poloxamer 188, 10% solution...adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...25513782. Pubmed Central PMCID: PMC4320685. 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post... OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun Tag (Viswanathan, 2015). The principal... -
Plasmids 101: Gateway Cloning
TypeBlog Post...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post... but now we’ll focus on how viral vectors have changed the scope and power of three commonly used behavioral... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post... PubMed PMID: 24226366. Lee, Min Woo, and Yinong Yang. "Transient expression assay by agroinfiltration... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post... first? They are starting by seeing if they can change long legged lizards into short legged lizards. ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...touch and reach back out when you are looking to change positions in the future. Anyone looking for work... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...targets (ones that are less likely to mutate or change in order for the bacterium to survive). Check out... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...may offer a lot of pointed feedback or abruptly change needs and expectations. Such situations can get...