Troubleshooting and Optimizing a Western Blot
Type
Blog Post
...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...reagent. Be aware that this approach requires excellent attention to detail, since you’ll end up with ...question before running one.
Good on gradients? Excellent! Now, let’s head west, intrepid reader!
Protein...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...to see if one works better than another. For nitrocellose membranes, you can even give gelatin a shot....strength
Sonication based lysing protocol
Subcellular fractional kit or lysing protocol
Not enough...