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  2. DNA Purification Without a Kit

    Type
    Blog Post
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...journal club should aim to have many of the same characteristics as a successful meeting: a clear purpose and...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

  4. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...and likely functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination...placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generation of the RNP complex.  While one option is to purchase the Cas9 protein and a gRNA from a commercial ...performed rapidly and, in many cases, with reduced chance for off-target effects. As the CRISPR field continues...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...college student searching couch cushions for spare change, desperate to extract a few more photons from a...Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits...

  10. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, ...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...of immunotherapy is the use of drugs (or other mechanisms) that work through body’s immune system to fight...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...during cell division. They don’t simply rely on chance (or random diffusion) to make this happen.  There...centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated by low-copy number...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...minutes before adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...ll notice that while the height of your events changes, the separation pattern of positive vs. negative...live population from my live-dead staining. (B) Changing the y-axis from SSC to FSC can alter the positioning...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another approach...
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