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We narrowed to 1,049 results for: ins

Showing: 761 - 780 of 1049 results
  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...
  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...or mentor. Several days prior to the interview, inspect your technology, background, and lighting for a...
  3. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...
  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...
  5. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...many journals use to verify whether a manuscript contains all the necessary information to be of maximal...
  6. Addgene: The First Twenty Years

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    Blog Post
    ...biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...
  7. Plasmids 101: Modular Cloning

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    Blog Post
    ...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...
  8. Viral Vectors 101: Chemogenetics

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    Blog Post
    .... The advantages provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects.... PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a...
  9. Tips for Technical Support Calls

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    Blog Post
    ...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within... Eric J. Perkins...
  10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...in a variety of outcomes including excision or insertion, inversion, translocation or cassette exchange...addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where...
  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... a STAR answer once the interview has started. Instead, I preferred to prepare stories to answer interview...possible. Otherwise, read the video conferencing instructions you received from HR carefully. And even if ...
  12. Plasmids 101: FLEx Vectors

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    Blog Post
    ... now specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx...optogenetic reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided...
  13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...light to distinguish one fluorophore from another. Instead of using a single (primary) detector to identify...narrow band, to discern one marker from another. Instead of compensation, which relies on user adjustment...
  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...
  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via...
  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...
Showing: 761 - 780 of 1049 results