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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,050 results for: ins

Showing: 761 - 780 of 1050 results

  1. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...

  4. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...many journals use to verify whether a manuscript contains all the necessary information to be of maximal...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...in a variety of outcomes including excision or insertion, inversion, translocation or cassette exchange...addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... a STAR answer once the interview has started. Instead, I preferred to prepare stories to answer interview...possible. Otherwise, read the video conferencing instructions you received from HR carefully. And even if ...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    .... The advantages provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects.... PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a...

  12. Tips for Technical Support Calls

    Type
    Blog Post
    ...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within... Eric J. Perkins...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... now specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx...optogenetic reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...

  18. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...plasmid of interest, for detecting the presence of an insert or piece of DNA in a sample such as in colony PCR...into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification. Following...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...
Showing: 761 - 780 of 1050 results