We narrowed to 971 results for: lars
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TypeBlog Post...This is, for the intrepid researcher looking for molecular biology tools, a very useful trait. Virus structure... Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered by the...
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CRISPR Library Amplification
TypeProtocol... Pooled Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly... LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...would take years and millions, if not billions, of dollars, but by approaching it from a new direction, we...Science Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived... -
DAP arrays for multiplex gene editing
TypeBlog Post...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology. https://doi.org/10.1002/jcp.30064... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ... -
K. phaffii: Rising to the Occasion
TypeBlog Post...evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you... -
5 Great Apps for Lab Life
TypeBlog Post... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...Protoctols for Your Research Catch Up On Your Molecular Biology Basics ... -
How to Lead a Great Meeting
TypeBlog Post... dialogue and community among participants, clarification - new and useful information, and invigoration...meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 minutes weekly... -
Fluorescent Proteins 101: Luciferase
TypeBlog Post.../Phototoxicity Not susceptible Susceptible Subcellular Imaging Improving Well-established High-throughput...effectiveness. Analyze Cell Signaling Pathways Known cellular signaling pathways can be investigated in a high-throughput... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...post-transcriptional gene expression across the majority of cellular pathways. Despite several years of research, the...pushed the field further with the creation of the largest miRNA sensor collection reported to date, containing... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post... through my work on the audit committee. I’m particularly curious to see how that progresses in 2019 and...Before you can go off and do great things in a particular area or profession, you need to make sure you... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post... this process, you will first need to do some molecular cloning. Depending on whether you are trying to...send your plasmid to a company. For a few hundred dollars, they will inject the plasmid into fly embryos ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization during ligand detection...