Cpf1 Update: Comparison to Cas9 and NgAgo
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Blog Post
... with gRNA sequence.
Both groups used multiple methods to examine Cpf1 off-target editing. First, they...
We narrowed to 850 results for: met
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Plasmids 101: FLEx Vectors
TypeBlog Post...allowing for rescued expression of GeneR. Sometimes also referred to as DiO (Double-floxed inverse... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post... terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...near your insertion site. Base editing, another method to create point mutations, also has a strict editing... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic... -
Of Myc and Men
TypeBlog Post...have been continuously pursuing new and better methods to peer into the mysterious, microscopic world ... -
Hot Plasmids: Winter 2025
TypeBlog Post...possible with conventional electrophysiological methods, it has remained difficult with GEVIs. Not only... -
Degrading DNA with Cascade-Cas3
TypeBlog Post... inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...the protein's behavior in cells, in solution or something in between, you are using a tag with a serious... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post... citation for you to use in your Materials and Methods and References sections when citing the use of ... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post... to include your improvements, or maybe even a methods paper. The problem with this is that it may take... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...their plasmids are available from Addgene in the Methods & Materials or elsewhere in their papers. Maybe... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post... any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated... -
An Introduction to Adenovirus
TypeBlog Post...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably developed... -
Lentiviral Vector Uses and Overview
TypeBlog Post... screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they... -
How to Write a Scientific Review Article
TypeBlog Post...This is the time to transform the document into something cohesive-- change the sentences, make it flow,... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... This surreptitious finding illustrates how the method used to select novel AAV capsid variants could ...