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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: mpl

Showing: 761 - 780 of 938 results

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...

  7. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible....

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

  10. New Coomassie Protocol Video

    Type
    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  15. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure...
Showing: 761 - 780 of 938 results