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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 996 results for: ran

Showing: 761 - 780 of 996 results

  1. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple of postdocs...

  2. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision repair, but ...but can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

  4. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...

  5. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

  12. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

  14. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  16. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...
Showing: 761 - 780 of 996 results