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We narrowed to 988 results for: ran

Showing: 761 - 780 of 988 results
  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...
  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...
  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...
  4. Plasmid Cloning by PCR

    Type
    Blog Post
    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...
  5. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...
  6. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...
  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...
  8. 5 Great Apps for Lab Life

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    Blog Post
    ...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS...
  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...
  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...example, matches of a name in the depositor field will rank higher than matches of the same name in the publication...
  11. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, ...
  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... a function in a given process. The process can range from a defined pathway or reaction to a complex ...phenotypical outcome. In forward screens, there is random or unknown manipulation of the population. This...
Showing: 761 - 780 of 988 results