Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 963 results for: tes

Showing: 761 - 780 of 963 results

  1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!    How to... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should be...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ...

  7. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette...

  11. Pipetting Protocol

    Type
    Protocol
    ...video for tips on pipetting in the lab. Equipment Pipettes Pipette tips Waste container Containers to hold...Tips Although there are many different types of pipettes that have different mechanisms to disperse liquids...liquids, this protocol is focused on single channel pipettes. These come in standard sizes: P2, P10, P20, P200...would read as 050. Each volume display on these pipettes will also have small tick marks at the bottom....Liquids of various viscosity have different flow rates. The more viscous a liquid, the lower the flow rate...’s important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense...

  12. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ..., VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 Pipettes, 10 mL, VWR 89130-898 Pipettes, 25 mL, VWR 89130...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...89130-900 Pipettes, 50 mL, VWR 89130-902 Gel loading tips, Corning 4853 Microcentrifuge tubes, Neptune 3745...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...neurons in the neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular...

  15. Five Popular Model Organisms

    Type
    Blog Post
    ...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ...

  17. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... for the majority of documented phosphorylation sites. If knowledge of a candidate regulatory kinase is...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...

  19. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ...and Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs...

  20. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Given the variety of these enzymes and the unique sites they recognize, restriction digests have become ...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...Analyzer , allow you to identify which restriction sites are present in a given sequence. For a list of the...either PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms... restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus making...
Showing: 761 - 780 of 963 results