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We narrowed to 955 results for: tes

Showing: 761 - 780 of 955 results

  3. Pipetting Protocol

    Type
    Protocol
    ...video for tips on pipetting in the lab. Equipment Pipettes Pipette tips Waste container Containers to hold...Tips Although there are many different types of pipettes that have different mechanisms to disperse liquids...liquids, this protocol is focused on single channel pipettes. These come in standard sizes: P2, P10, P20, P200...would read as 050. Each volume display on these pipettes will also have small tick marks at the bottom....Liquids of various viscosity have different flow rates. The more viscous a liquid, the lower the flow rate...’s important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense...

  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... for the majority of documented phosphorylation sites. If knowledge of a candidate regulatory kinase is...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...

  6. Five Popular Model Organisms

    Type
    Blog Post
    ...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments...

  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ...and Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs...

  8. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...neurons in the neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ...

  11. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ..., VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 Pipettes, 10 mL, VWR 89130-898 Pipettes, 25 mL, VWR 89130...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...89130-900 Pipettes, 50 mL, VWR 89130-902 Gel loading tips, Corning 4853 Microcentrifuge tubes, Neptune 3745...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly...

  13. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Given the variety of these enzymes and the unique sites they recognize, restriction digests have become ...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...Analyzer , allow you to identify which restriction sites are present in a given sequence. For a list of the...either PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms... restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus making...

  14. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...molecular biology, plasmid cloning, and titering and testing your viral preparations. Addgene...and ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add... mouse genes. Virus Protocols for titering and testing your virus preparations. Name Description Link ...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature Methods, 2(4..., G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot,...

  16. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can ... cartoon, in which we are adding EcoRI and NotI sites to Your Gene of Interest (YGOI) for ligation into...amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis tool, such as Addgene...Analyzer , to allow you to identify which restriction sites are present in a given sequence. You want to choose...based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1...

  17. Modular Cloning Guide

    Type
    Guide
    ...sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose from...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...protein complexes in various hosts. Expression cassettes for individual subunits are first assembled by...

  18. Protocol - Bacterial Transformation

    Type
    Protocol
    ... on ice (approximately 20-30 mins). Remove agar plates (containing the appropriate antibiotic ) from storage...that there isn't too much liquid media on the agar plates. If the agar plate doesn't dry adequately before...the liquid and won't grow in colonies. Incubate plates at 37°C overnight. Tips and FAQ How can I save ...access to an electroporator and the appropriate cuvettes. Follow the manufacturer's instructions for each...

  19. Lentivirus Production

    Type
    Protocol
    ...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ... plate in DMEM Complete in 10 cm tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h...the table below for a possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL of 1 mg/mL PEI 1:...Centrifuge the viral supernatant at 2100 rcf for 5 minutes to pellet any packaging cells that were collected...

  20. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes Benchtop centrifuge compatible...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...Reagent Preparation instructions. Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent...
Showing: 761 - 780 of 955 results