We narrowed to 1,054 results for: tra

...Novophage's successful transformation into Sample 6 and Dr. Koeris' own transition from graduate student... why? 24:41 - 29:00 : Dr. Koeris compares and contrasts being a graduate student with being an entrepreneur...37-36:53 : Was It difficult for Dr. Koeris to transition from developing therapeutics to developing a ...

... °C but preferentially nicks the non-target DNA strand. The lab then found gain-of-function mutations ...mutations that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature... of stable genomic integration and is easily transferred via conjugation.   Read the paper in Nature...plasmids CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method...

...many tables were set up with various science demonstrations, and students could walk around and ask scientists...to convince me to spend a Saturday in the tourist-trap nightmare that is Times Square. But I made the sacrifice...we talked.  That's a win. Mark Lucera is a PhD trained scientist at the University of Colorado, Anschutz...Jennifer Stancill I am a research biologist by training, so of course I felt compelled to participate ...name is Erin and I have spent the last 15 years training as an academic scientist. I’ve worked tirelessly...

...gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with...base editor variants using a protein-truncation strategy with reduced RNA-recognition capabilities.  Read...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...

...vacation in August (for another part of my job, I travel around the country and set up meetings with PIs...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...Epigenomics 5 Tips for Troubleshooting Viral Transductions Single Base Editing with CRISPR Cas9 Activators...

...PubMed Central PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse...

.... As our organization grew, we continued this tradition. We now have over 30 full-time employees, so we...baked goods and we have a few unique lunchtime traditions that might be fun to incorporate into your own...think these random lunches would be a wonderful tradition for university departments to establish, with ... my Addgene family every day. Do you have any traditions that you share with your labmates, be they food...

...figure). Most of the mutations were found to be “neutral,” having no significant effect on cell viability...drivers of tumor formation. Being able to parse out “neutral” passenger mutations from the “active” drivers ...(2018): 450-462. PubMed PMID: 29533785. PubMed Central PMCID: PMC5926201.  Tsang, Yiu Huen, et al. "Functional...7 (2016): 10500. PubMed PMID: 26806015. PubMed Central PMCID: PMC4737758. Additional resources on the ...

...professional-grade design software, like Adobe Illustrator, and I didn’t want to pay for these programs ... Vectr is like a pared down version of Adobe Illustrator. There’s both a web and a desktop version of ...Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for drawing your own...20,000 icons is designed by professional medical illustrators with input from life scientists. It’s easy to...

...not possible to elicit an immediate response or track the exact time of activation. Receptor activity ...turned off. In chemogenetics, while different concentrations of ligands can be injected, this is not as ... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

... Being an SME is often like being a jack-of-all-trades. “As a subject matter expert in the educational...testing those experiments. “We also do videos demonstrating an experiment or a technique to students, like...departments at eScience Labs. This can involve traveling with the sales team to meet faculty and introducing...open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens.” And since...

... tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at elevated ...Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9 or SpCas9 RNP’s and... in diluted human plasma for 8 hours at 37°C. Substrate was then added and enzyme activity measured. SpCas9...reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on...

...equals, because as soon as I had passed my training, I dove straight into my capstone project. A capstone project...well as complete the lab safety and cybersecurity training before stepping foot into the lab itself. I appreciated... had made a mistake by not having the right concentration of a reagent during our HiFi assembly but decided...

...conventional antibodies include:  Excellent tissue penetration, thanks to their small size of 12–15 kDa Ease...sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs in the clinic sdAbs have...also been some interesting work done developing transgenic mice that express camelid variable domains (aka...2021) Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https...

...course in the English department wouldn’t have trained me in the scientific aspects of writing. Following... discussed individual sections of a paper, the abstract, figures, introduction, etc. so as to thoroughly...manuscripts as well as being critiqued added an extra level of application not present in the online class...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

...and teaching community through the TRAINED initiative (Translating Research on Arabidopsis Into a Network...teachers’ preference for using animal systems to demonstrate biological concepts - and you have yourself a...experiments that include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with...

... available materials is growing along with a centralized information resource for scientists studying ...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited...could keep up with demand and keep new projects on track to succeed at the same time. I began approaching...needs of scientists worldwide with Addgene’s traditional excellence through whatever lies ahead this year...

...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...enjoyed a taste of what was to offer, continued straight on to a master's degree in science communication...venturing forth from tertiary study, the myriad trajectories ahead can be condensed into two discernible ...stroll from my office to the tearoom would often illustrate the breadth of knowledge and passion on display...

...experimental details (e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid...helpful because viewers will know exactly which strain you used so if they wish to replicate your experiment...experiment, they know exactly where to get that strain. Experimental design & methods: These questions are... Using AAV Technologies Using AAV for neuronal tracing Resources on Addgene.org Read our AAV guide Browse...

...ordering viral vectors. Virus production can be a frustrating process that requires a large investment of time...have experience with it. We’re reducing this frustration and getting researchers to the experiments they...applications where we have delivered them in vivo to the central nervous system. This service will further enable... titer lentiviral preps, troubleshoot viral transductions, and overviews for beginners just starting work...