Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 210 results for: FAST

Showing: 31 - 40 of 210 results

  1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor Lower-stringency...resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct...The idea was to evolve a TadA-7.10 version with a faster deamination rate so that deamination can happen...PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems allow for rapid continuous...speed dictates which phage variants can propagate faster than the rate of dilution (passage from the infected..., they identified TadA-8e, which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 ...functional domains speed up the development towards faster and more precise base editors capable of editing...

  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...CRISPR plasmids New items from the viral service A fast blue-light inducible Cre for precise control of ... can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.  Sheets, MB...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...

  7. CRISPR Cheat Sheet

    Type
    Blog Post
    ...CRISPR material for you!  The CRISPR field moves fast. That’s why we’re bringing you a brand new third...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... to grow, clone, and manipulate their vectors in fast-growing bacteria first. In fact, most yeast vectors...you also have to consider how to keep it there. In fast-growing populations of cells, the vector needs to...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...
Showing: 31 - 40 of 210 results