We narrowed to 93 results for: aav gfp cre
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TypeCollection....hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...sensor, Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-...
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Retrograde AAV viral preps
TypeCollection...Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control...Gradinaru 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 24593 AAV-pgk-Cre PGK Cre expression ....SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...GCaMP7s, Cre-dependent Calcium sensor Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent...104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s, Cre-dependent Calcium sensor Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE... -
Control AAV Preps
TypeCollection...Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10... not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Sanes...Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP...-EGFP CAG EGFP Cre dependent 1 Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru... -
Optogenetics AAV Preps
TypeCollection...Deisseroth 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato...soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive 9 Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn...ArchT tdTomato Cre dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive...-rc [Jaws-KGC-GFP-ER2] CAG Jaws GFP Cre dependent 1, 5, rg* Boyden 84446 pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2...Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 ...Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Boyden 59171 pAAV-Syn-ChrimsonR-tdT...Deisseroth 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Boyden 84445 pAAV-CAG-FLEX-rc... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and clonogenic...strain in which Cre expression causes the induction of both DsRed fluorescence and GFP shRNA. Mice carrying...putida and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on...CRISPR/Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists to validate ...adeno-associated viral (AAV) system enables greater spatio-temporal control over expression and increases the number... -
Brain Armamentarium
TypeCollection...Ting Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...PHPeB AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression...AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase...PHPeB AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...injury markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs...Testing AAV-SaCas9 in vivo To test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-...together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this...co-infection of two AAVs. Cas9 orthologs: shorter, but just as potent and specific? The two AAV strategies described...proteins with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within AAV’s packaging...in length, allowing them to be packaged in AAV. Split AAV approaches While finding smaller Cas9 orthologs...that the field has overcome with split AAVs. In general split AAVs break a large transgene in two pieces... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...Introduction to AAV Find AAV Calcium Biosensor Vectors at Addgene ...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been... tissue-specific targeting. By employing various AAV serotypes, researchers can precisely direct the calcium... ready to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle...3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’... aav... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the... the brain of Cre-expressing mice to trigger recombination and expression of a transgene (Lin et al., ...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...site upstream of the stop cassette results in decreased efficiency of recombination and preferentially... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post... Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral ...retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can...the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408) This ...lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments...Vectors Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression. Cloning and expressing RNAi pMKO.1 puro (Plasmid...of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, and selection...