We narrowed to 144 results for: transfection

...brain? Cells of a specific brain area can be transfected by in utero electroporation. To achieve sparse... could reduce the plasmids amounts to reduce transfection efficiency. However, if the cellular structure... it reduces the probability that neurons are transfected with both vectors. Flex vectors are the solution... FLEx vectors guarantees that most cells are transfected with all labeling plasmids and the low concentration...

...transient cotransfection of the mARG plamsids into HEK293T cells using a chemical transfection reagent ...proteins. Excess gvpA plasmid added into the transfection mixture in HEK293T cells gave drastic improvement...These plasmids can be used directly in transient transfection in rtTA+ cell lines (such as HEK293-TETOn cells...visible under phase contrast starting day 2 after transfection.     Fig: 2: Expressing mARGAna in MDA-MB...they can also be integrated into the genome by cotransfecting them with a plasmid expressing PiggyBac transposase...saturate expression. When engineering a cell line, cotransfect the mARG cassettes 2:1 (A:N-V) with PiggyBac ...reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-...

..., you’re ready to edit. These cells are then transfected with several different constructs for the reporter... gRNAs by simply changing the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The...plasmid.  With this set up, all successfully transfected cells will express RFP while cells containing...

... strategy for protein expression consists of transfecting cells with your DNA template of choice and allowing...SV40, CMV, and RSV) for robust expression post-transfection. Mammalian systems can express proteins both... Both methods produce high protein yields if transfection is successful. Some mammalian systems also ...

... how lentiviral particles are produced by co-transfecting three essential ingredients: the lentiviral ...the 3rd-generation system is that it involves transfecting four different plasmids into the producer cells...use lentiviral transfer vectors for transient transfections? A2: Technically, yes. Will it actually work...

...details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via...

...sequencing to confirm library representation. Transfection of the DNA pooled library and appropriate packaging...Addgene.) This small aliquot is not enough to transfect producer cells, so your first step will be to ...in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching PubMed ...

...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...

...constitutive promoter can be used to monitor transfection or viral infection efficiency, to study changes... Renilla luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623... expressing firefly luc for monitoring plant transfection. Daniel Voytas 133312 pMKV057 Firefly AtUbi10...and IPT, with firefly luc for monitoring plant transfection. Daniel Voytas 181934 pC0037 Gaussia, Cypridina...

...replication. To generate a virus, you need only transfect the ‘helper’ plasmids (viral packaging machinery...Protein production  HEKs are extremely easy to transfect in bulk and produce recombinant proteins at high...