We narrowed to 160 results for: ALC
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TypeBlog Post...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...
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Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ... -
AAV Viral Preps
TypeCollection...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls... -
Antibody Production
TypeCollection...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software... -
Fluorescent Protein Guide: FRET
TypeCollection...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA... -
Rett Syndrome
TypeCollection...Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link opens in a new window... -
2021 Deck the Lab Winners!
TypeBlog Post...December 10, 2021 2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng... -
Deisseroth INTRSECT Collection
TypeCollection...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used to generate... -
Deck the Lab 2022!
TypeBlog Post... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing... -
Celebrating One Million Plasmids Shared!
TypeBlog Post... This massive scale reagent sharing led us to calculate how much time we have saved researchers. In a ... -
Fueled by Coffee at #SfN14
TypeBlog Post... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...aequorin, gives off a faint blue light when it binds calcium ions, and the second, which we now call GFP, absorbs... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year ... -
Editor's Choice, September 2016
TypeBlog Post... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments... -
Sensing Neuronal Dopamine
TypeBlog Post... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...