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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 65 results for: lentivirus production

Showing: 61 - 65 of 65 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... repeat. RRE Rev response element. A.3 Related Products The following plasmids available from Addgene ...Incubate at 37°C for 1-2 hours. 4. Run the digestion products on a 1% agarose gel. You should see two fragments...obtain the ratio that gives you the optimal viral production. d. Create a master mix of FuGENE® 6 transfection...DS et. al. Cell . 2003. 115:199-208. PubMed . Lentivirus-delivered stable gene silencing by RNAi in primary...1997. 15(9):871-5. PubMed . Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry...

  4. CRISPR Guide

    Type
    Guide
    ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...G-to-T:A base editors with higher efficiency and product purity. Science Advances . 3 (8), eaao4774. PMID...

  5. Plan Your Experiment

    Type
    Guide
    ...more likely to result in a non-functional protein product. Be aware that there could be downstream translation...more information about viral vectors and their production, see our viral vector guides . Lentiviral vectors...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with...
Showing: 61 - 65 of 65 results