We narrowed to 160 results for: ALC

1. Addgene Depositors Get More Citations

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   ... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

2. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...

3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   ...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...

4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...

5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...

6. Screening for Successful Genome Editing with Digital PCR

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   ...wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ...

7. CRISPRainbow and Genome Visualization

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   ...physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously...

8. Hot Plasmids February 2024

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   ...doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains...

9. Viral Vectors 101: Chemogenetics

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   ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...

10. Viral Vectors 101: Virus Safety

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    ..., which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine ...

11. Fluorescent Proteins 101: Introduction to FRET

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    ..., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

13. Hot Plasmids and Viral Preps - March 2021

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    ...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

14. Hot Plasmids - October 2020

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    ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

15. Pooled Library Amplifications

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    ...transformation efficiency, or more specifically calculated based on the size of and weight of your library...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed...

18. Light Sheet Fluorescence Microscopy

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    ...Spalteholtz (10).  He used a solution of benzyl alcohol and methyl salicylate which was later modified ...

19. Penn Vector Core Partnership with Addgene

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    ... (including DREADDs) Optogenetics Recombinases Calcium Sensors and other Biosensors Controls To look up...

20. Tips for arabidopsis transformation

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    ...enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options...

21. DNA Purification Without a Kit

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    ...proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA...

22. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...

23. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...guide RNA sequence you used. The ICE tool will calculate overall editing efficiency and determine the profiles...

24. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...measured by qPCR.   Physical titer is typically calculated by two popular PCR-based methods: Quantitative...

25. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...

26. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ... later steps in this chain (blue) to infer or calculate things about earlier ones. In practice, multiple...

27. Hot Plasmids: Summer 2024

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    ... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like...

28. CRISPR 101: Validating Your Genome Edit

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    ...clones from that population. You can additionally calculate the minimum number of clones you would have to...

29. Harnessing Bacterial Toxins for Allelic Exchange

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    ...synthetic ribosome binding site (using the online calculator from the Salis group) (Espah Borujeni et al.,...

30. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...and determine the topology of the mitochondrial calcium uniporter (Martell et al., 2012). It is important...