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We narrowed to 379 results for: Lor

Showing: 91 - 120 of 379 results

  1. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... and treatment of cancer have been extensively explored. Biologists have attempted to study cancer from...ve been studying it and, now, researchers are exploring ways to bolster and modulate our immune defenses...bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms -...efficient outcomes, collaborative therapies must be explored. When attacking cancer, multiple immunotherapy...and cancer immunotherapy, and is interested in exploring new avenues in healthcare.   References 1. Vinay...

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies ...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s ... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

  3. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...and boost your productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your...For binders of protocols and data, consider using color coded binders and tabs (for different experiments...notebooks and go wild with sticky notes and labels. Color code your Post-its and write brief descriptions ... the notebooks, so that you can easily spot the colors and text. Tip 2: Use electronic to-do lists to ...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012... monitor protein-protein interactions through multicolor labeling and FRET. Specialty constructs for biosensing...e.g., EGFP and YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable...at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer a comprehensive...

  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University, and the Karolinska...protein CFP484. Collaborating with scientists from Florida State University, they used a mutagenesis approach... max emission at 509 nm), making it useful in multicolor labeling particularly with fluorophores that ...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology...

  6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...another gene controlling eye color) and yellow (a gene affecting the body color of the adult fly). This is...such marker is the white gene, which restores eye color to fly lines where the endogenous white gene is ...anywhere from light yellow to red. The variation in color depends on how transcriptionally active the genomic...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose...galactose and an insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but how does this help us...β-galactosidase, and remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase...increases pigment precipitation, enhances the blue color of negative colonies, and allows for better differentiation...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website...please visit the Cell Catalog on the Allen Cell Explorer, where you can also find the certificate of analysis...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool, and develop new...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...York Lab operates with a strong commitment to exploration and collaboration. Andrew is a physicist with...inventors and biomedical engineers, join efforts to explore the mechanisms and develop potential applications...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration must be used ...and/or different IgG subclasses of 1° for multi-color staining Can be expensive Nanobodies   Recombinant...staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don’t require...application to the sample. Staining with this multi-color staining workflow yields similar localization patterns...

  11. Bacterial Expression Systems

    Type
    Collection
    ... pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Garnett 3rd 2 37,722 Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green ...

  13. Deep Dive: qPCR

    Type
    Blog Post
    ... or greater with good statistical significance (Taylor, 2017). With the right conditions and parameters...):332-7. doi: 10.2144/00292rv02. PMID: 10948434.Taylor SC; Laperriere G;  Germain H. Droplet Digital PCR...2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; Nadeau, K; Abbasi, M; Lachance, C; Nguyen,...j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...did perform similarly to GRABNE, and having both colors allows for increased options for multiplexing in...Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo...Microscopy Technique is Best for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources ...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... a powerful tool to modify mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru... School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous...2015. PubMed PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques...colleagues at Osaka University have developed two new color variants of their Nano-lantern technology, first...emission spectra suitable for single cell, multi-color imaging. The Nano-lanterns rely on the principle...monitoring multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai...

  17. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors Loren Looger New serotype pAAV-CAG-FLEx-iSeroSnFR AAV9...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...B978-0-12-800947-5.00004-1 Taylor, M. W. (2014). What Is a Virus? In M. W. Taylor (Ed.), Viruses and Man:...are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t even decide...

  19. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    .... They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell behavior...GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations responsible...disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers found...

  21. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... where 1000 years ago the hero lost and the Dark Lord rules all, the books center around a cast of endearing...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

  22. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...Here, we look at the origins of the system and explore advances that have happened since our original ...of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia Research Campus to ...affinity for glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR...

  23. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...single mRNA over time. Both are helping researchers explore the complexity of translational control in cellular... and translated RNAs appear red. To enable this color switching, TRICK requires the following components...translocation rate of ~3.5 codons/second. They also explored the effects of 5’ UTR variants in translation ...

  24. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...services! Many thanks to Joe Mellor, our guest writer from seqWell Inc. Joe Mellor is the Founder and CEO of...This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR...

  25. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...BioBuilder embraces new opportunities for scientific exploration, discovery, and collaboration. One module currently...and the new BioBuilder module allows students to explore how engineering fields rely on rapid prototyping...the lab as a starting point into further STEM exploration.  Further offerings for both STEM teachers and...

  27. Antibodies 101: Conjugation

    Type
    Blog Post
    ...antibodies that produce detectable signals like light or color. In this post, we’ll go over some of the common ...blots and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...filtration.    There is, of course, a lot more to explore in the world of antibody conjugation. But hopefully...

  28. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Rosella fluorescent properties Rosella is a dual color-emission biosensor named after the brightly-coloured... or lysosome. See Table 2 for a summary of what color fluorescence Rosella emits based on its localization...fluorescence emission. Localization pH Emission Color Cytosol ~7.5 Red and Green Mitochondria ~...

  29. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...all the plasmids from the associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2...ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular fluorescence complementation...(split HaloTag) provides maximum flexibility in color labeling with exogenous fluorophores, which can ...

  30. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...over the past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR)...solutions. The 2015 Boston FoR meeting was designed to explore areas where further data is necessary to determine... 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost generation ...
Showing: 91 - 120 of 379 results