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We narrowed to 273 results for: aav

Showing: 91 - 120 of 273 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...optogenetic experiments. Intracranial injections of AAV are commonly used to deliver channelrhodopsins to...collected following intracranial injection of an AAV expressing a ChRger. In both experiments, neurons...Designer channelrhodopsins paired with a systemic AAV allow for minimally-invasive optogenetics While ChRgers...delivered ChRgers by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of the nervous... on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about the...

  2. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays...platforms: transfection, lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments...array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes in a ...experiments, cells were infected with a 1:1 ratio of two AAVs,  one expressing Cpf1 and the other a crRNA array...Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... it is too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially...archaea are much smaller and would be ideal for such AAV packaging, but their activity in mammalian systems... Cas12a based systems, making it well suited for AAV packaging. Most importantly, this CasMINI system ...effective at genome editing when delivered in an AAV system, and demonstrated that the new system was ...methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical Biology Find...

  4. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known to cause...integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety...safety level. Adenovirus is similar to AAV in that it does not integrate into the host’s genome. However...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

  5. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...Collection Addgene's AAV Data Hub More resources on the Addgene blog Newly Updated AAV Data Hub Antibodies..."Pass". Curating the data reports Just like our AAV Data Hub, each data submission we receive is checked...

  6. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...also rolled out an updated AAV Data Hub to share curated information about AAV usage in research applications...applications. Scientists around the world can upload AAV validation data, sharing what works and, equally importantly...

  7. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of...the image background.     If you’re working with AAV or lentivirus in your research, you might want to... and needed a break so they're hanging out with Aavery in this Zoom video background. Want the purple ...bacteria video background with mascots Blugene and Aavery? Download the 720p version or download the 1080p...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from ...in serotype AAV5! Get dLight1.3b here Looking for a knock-out in your experiments? AAV-flex-taCasp3...available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin...SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan...the Deisseroth viral preps here Coming soon! New AAV serotypes from the Sanford Boye lab with enhanced...include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8...Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature...

  9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable...for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9 (packaging...or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery....hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing Dividing...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future...experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV Data Hub to help the research...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

  13. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Gether lab, now available as ready-to-use AAV vectors.   ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether...

  15. Reflections and Looking Ahead

    Type
    Blog Post
    ...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed...

  20. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider...

  21. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once...

  23. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research... excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of...

  24. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned...

  25. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...

  26. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

  27. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request  ...lab has a limited quantity of AAVs that can be requested by contacting Alexander Lohman ([email protected]... T7 pAAV-hSYN1-NES-OCaMP-WPRE pAAV hSyn pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE pAAV Ef1a ... pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab...

  30. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our...
Showing: 91 - 120 of 273 results