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  1. Viral Vectors 101: Inverted Terminal Repeats

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    ...Transgene Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. ... System. Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288....capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775...Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513....increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345...

  2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    ...identifier format for lenti-Cas9-VQR is the URL “http://n2t.net/addgene:87155” where the Addgene registered... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” ...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite... gift from Daniel Bauer (Addgene plasmid # 87155 http://n2t.net/addgene:87155)” Cite plasmid using RRID...

  3. Hot Plasmids - May 2022

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    ... 2021. https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature Neuroscience. 2022. https://doi....  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP...plasmids here! Wang et al. Nat Cell Biol. 2022. https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes...barcode plasmids here! Kaufman, et al. 2022. Nature Comm.https://doi.org/10.1038/s41467-022-30008-0       ...

  4. Antibodies 101: Monoclonal Antibodies

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    ...additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080/19420862.2018.1445456  Holzlöhner...Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, ...Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari...Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942  National Research...Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov/books/NBK100203/ Additional...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical ... and Specificity. JACS 144, 12584-12594 (2022). https://doi/pdf/10.1021/jacs.2c02633 Allen, D., Rosenberg... Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu... gRNAs. Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori...CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More...

  6. Antibody Validation for Flow Cytometry

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    ...major research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende...immunofluorescence and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme...ALS/FTD disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski...of antibodies. Nature Methods, 13(10), 823–827. https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle...British Journal of Pharmacology, 178(15), 2948–2962. https://doi.org/10.1111/bph.15467 Virk, H. S., Rekas, ...of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019-55133-7 More resources...

  7. Design Tips for Prime Editing

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    ...breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J.,...editing outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. ...efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional ...

  8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...factors modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD...Protein for Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith...prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam...recognition of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna... Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources...

  9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...in Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J ...the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De...Single-Domain Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann ...delicate proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I...conformational trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources...

  10. Of Myc and Men

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    ...proto-oncogene product. Mol Cell Biol 5:3610–3616. https://doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro ...domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984.tb02263.x  Munro...immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, ...secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner...on neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional ...

  11. Light Sheet Fluorescence Microscopy

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    .... Pubmed. Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab.../keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...ultramicroscope-overview.html http://www.zeiss.com/microscopy/en_de/products/imaging-systems/lightsheet-z-1.html ...

  12. Plasmids 101: Stringent Regulation of Replication

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    ...Fundamental Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & ... RNA site. The EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar, G....Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade...replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., ...copy number. The EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources at ...

  13. Using Video to Share Your Science: We Share Science

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    ...Video on We Share Science: http://wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology... on We Share Science: http://wesharescience.com/pin/7439 Research Website: https://umaine.edu/aquaculture...current-biology/abstract/S0960-9822(16)30873-9 Youtube: https://youtu.be/EHHhiawIdWM Saving salmon, one embryo ...

  14. Antibodies 101: Validation

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    ...Blame it on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun...Standardize antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, ...suitable antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig...view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...

  15. Plasmids 101: Using Transposons in the Lab

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    ... Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448...transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez...Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871 Pray, L. (2008...: The jumping genes. Nature Education 1(1):204. https://www.nature.com/scitable/topicpage/transposons-... and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources...

  16. Antibodies 101: Introduction to Antibodies

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    ...Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora...expression: The nuts and bolts. Methods 116:51–62 . https://doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-...elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091 Cruse JM, .... In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang ...chaperone Skp. Front Cell Infect Microbiol 3: . https://doi.org/10.3389/fcimb.2013.00072 Additional resources...

  17. CASTing Off for New Shores in Human Genome Editing

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    ...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. ...RNA-guided DNA integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E...United States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., ...CRISPR-associated transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources...

  18. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    ...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...  Zalatan et al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids...described in Qi et al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources...characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-Toledo, J...

  19. Hot Plasmids - February 2022

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    ...Genes Cells. 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038...domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered...therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

  20. GPCRs: How Do They Work and How Do We Study Them?

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    ...human GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, ...Peroxidase-Catalyzed Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016...DREADDs for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S,...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  21. RNA Extraction Without A Kit

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    ...Biophysica Acta (BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin ...enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi...Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski...twenty-something years on. Nature Protocols 1:581–585. https://doi.org/10.1038/nprot.2006.83 Additional resources...

  22. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...the National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa...epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J...DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL,...Purification. Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional...

  23. Plasmids 101: The Wide World of Natural Plasmids

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    ...and Environmental Microbiology, 67(8), 3440–3444. https://doi.org/10.1128/AEM.67.8.3440-3444.2001 Fogarty... in the human gut. Cell, 187(5), 1206-1222.e16. https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia... Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684 Shareck,...Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org/10.1080/07388550490904288 Additional Resources...

  24. Hot Plasmids and Viral Preps - January 2021

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    ...hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian...’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome...from CRISPR-Casɸ. Pausch et al., Science 2020, https://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the ...molecules.  Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner...

  25. Production of Virus in Insect Versus Mammalian Cells

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    ...HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, ...Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., ...2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. ...lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

  26. Hot Plasmids and Viral Preps - May 2021

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    ... Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126...Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein...biosynthetic pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR...

  27. Tips for Using BLAST to Verify Plasmids

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    ... BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services...services http://www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk...

  28. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...artifacts and how to minimize them. FocalPlane. https://focalplane.biologists.com/2020/07/07/fixation-...Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177/33.8.3894499    Im K, Mareninov...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26  Rolls G ...Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com/us/knowledge-pathway/fixation-and-fixatives...

  29. DNA Purification Without a Kit

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    ...Purification Method Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein... of the United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason...and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

  30. Hot Plasmids - August 2020

    Type
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    ... Ting. Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing ... Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a ...purification. Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed...plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR...
Showing: 91 - 120 of 254 results