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We narrowed to 968 results for: Abo

Showing: 121 - 160 of 968 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

  13. AAV ddPCR Titration

    Type
    Protocol
    ...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...

  14. CRISPR Library Amplification

    Type
    Protocol
    ...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...

  15. Addgene's eBook Collection

    Type
    Guide
    ... | Fourth Edition About Plasmids 101 September 2023 | Fourth Edition About Based on our popular...techniques! Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January...screens. Close About Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent... more! Close About Overlay Select Viral Vectors 101 July 2024 | Second Edition About Viral Vectors...more! Close About Overlay Select Science Career Guide November 2018 | First Edition About Science Career...Close About Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR... trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay ...

  16. Antibody Guide

    Type
    Guide
    ...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

  17. Chemogenetics Guide

    Type
    Guide
    .... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics Guide . ...tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors...

  18. Plan Your Experiment

    Type
    Guide
    ...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...controls when working out a new protocol. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...and Proteins In addition to the methods described above, you can also directly deliver mature mRNA or purified... approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will...Browse Cas9 plasmids for RNP delivery . As mentioned above, CRISPR efficiency will vary based on the method...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods ....

  19. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...increased safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus...packaging methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected... Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L.,...

  20. Molecular Biology Reference

    Type
    Guide
    ...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...codon. Below you will find helpful resource tables about the genetic code. This table includes the nucleotide...

  21. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors...gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's gamma-retroviral...are necessary for lentiviral production in the laboratory, and many have been removed or mutated for increased... genome. To produce lentiviral vectors in the laboratory, the non-required components have been removed...

  22. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...our adeno-associated viral vector guide to learn about AAV vector components, production and common uses...E2a and VA for replication. For more information about adenovirus, read our Adenoviral Vector Guide . Recombinant... factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog post on ...in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics 101 video...

  23. Educational Resources

    Type
    Guide
    ...biology, laboratory research, and career development. eBooks Download any of our eBooks to learn about popular...Playlists include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes...

  24. CRISPR Guide

    Type
    Guide
    ...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

  25. Adenovirus Guide

    Type
    Guide
    ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...E2a and VA for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral...linearized construct, and rAdV vectors can be harvested about 7–10 days later. Vogelstein designed multiple shuttle...

  26. Science Guides

    Type
    Guide
    ...the following guides to help you start learning about various scientific concepts related to our plasmid...Viral Vectors Find educational guides to learn more about the different types of viruses that are commonly...

  27. Guide to Using Pooled Libraries

    Type
    Guide
    ... to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used. Below...simultaneously in a single reaction. As discussed above, access to this technology is crucial for conducting...

  28. Promoters

    Type
    Guide
    ...of interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

  29. Optogenetics Guide

    Type
    Guide
    ... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

  30. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...keep spreading the word about materials sharing, but we don’t have to worry about getting support from depositors...of course always being vigilant about resources, I’m not worried about the stewardship of this in the ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...on this. Addgene’s success is about the Addgenies. I never worry about their dedication, excellent intentions...more about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our ...a lot of things we actually don’t have to worry about and that is something to recognize! Distributing...the last couple of years, we don’t have to worry about having enough space! There’s enough space here at...

  31. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...topics many times, but I always seek out information about the particular audience and venue before tailoring...

  32. PhD Applications After COVID

    Type
    Blog Post
    ...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...professors will not only talk about their own interests, but will ask you questions about your research goals and...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...this:   However, as simply as it seems laid out above, there are many places where the PhD application...-hand, you can quickly reference your questions about the PI’s research or the name of the award you won...new way to allow you to get important information about the campus even if you can’t visit. By talking with...department, the lab setting, and how students truly feel about the program and faculty. Here are some questions...

  33. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... because we are highly collaborative, we love teamwork. Working collaboratively allows our team to best...clarification and again we collaborate with the Product Team who in turn collaborates with stakeholders to ...Scrum framework is based on an iterative and collaborative approach to development. Scrum teams plan and...that it provides transparency to everyone involved about the status of each project. This creates accountability... amongst the team. Whether we are a highly collaborative team because we are using the Scrum framework...These initial stories are usually a result of collaborations between Addgene’s Product Team and the stakeholders...Once we begin work on a story, we continue to collaborate closely with each other and with other teams....

  34. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...dig deep Talk to your professors about their research, and learn about what makes them excited to do what...sciences), people conduct research to answer questions about how the world works. As scientists, we probe beyond...curious person. I like to ask questions and think about the best way to answer those questions. I’ve met...enjoy, is worthwhile and can help us make decisions about what we want to do in the future. How do I get started...sense of what kind of research you would be excited about. I know quite a few friends who opted to join labs... a lot out there worth exploring! Being excited about the science allows you to ask more interesting questions...as graduate students (your teaching assistants), about their experiences. Research requires dedication ...

  35. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...matters Yes, yes it’s all about the science but really it’s not. It’s about the atmosphere that will allow...and are nervous about discussing data. There’s hesitation before answering questions about lab culture. ... emotions and assumptions. I’m not just talking about simple things like “Which route should I take to... and not very communal. That didn’t suit my collaborative learning style at all. Finally I rotated in ... did). Some of the information you will need is about you. It’s worth taking time to honestly evaluate...lab members can give open, revealing information about the culture and lab head. While it’s good to talk...same department as well. What do they have to say about this lab’s reputation? If you are choosing a training...

  36. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about the...ot­her disciplines, are even less likely to read about our work. We therefore must discover new ways to.... Only on occasion do we then have time to read about research from other disciplines, or from other countries...communication through video     Video abstracts about research, especially published research, have grown...researchers have Facebook pages, few share any details about their research itself. Pinterest is also a favorite...created a new platform for sharing video abstracts about research: www.WeShareScience.org. WeShareScience...platform for sharing short, 3 to 5 minute videos about research. Videos can be shared by research scientists...

  37. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...possessed. Think about your shining moments. Recall a few times you really felt great about your work. Maybe...having a life outside my job. The same can be said about teamwork, communication, writing, management, and...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...add value, not just “do the job”. When they ask about your creativity, your potential manager wants to...outside the box to solve a problem. When they ask about your communication style, your potential teammates...they have developed as part of a successful collaboration and fail to identify it as a personal strength...yourself in situations where you can learn more about possible strengths and weaknesses, and view everything...

  38. Lab to Office Culture Shock

    Type
    Blog Post
    ...scientist anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at ...10 years, but when people ask me about my job, I have no qualms about saying “I’m a scientist.”    What...installment of the Addgene career guide, we’ll think about what causes this culture shock and how to overcome...counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues, we’ve...This is probably because you don't have to worry about experiments taking longer than expected or dealing... hour days at the lab, I encourage you to think about how much of that time you’re actually spending doing...candidates over the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is ...

  39. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...experiments (and editors) can move slowly, news about possible broad changes to policies seems to break...primarily by wishful thinking. The March for Science is about fighting scientific partisanship, including more... could walk around and ask scientists questions about their work. Thousands of people showed up; some ...justice. Whether it’s used to fight the myths that abortions cause breast cancer and infertility or to argue...reproductive age live in a state with at least two abortion restrictions that conflict with scientific evidence...Mark Lucera I am a passionate scientist.  I care about my work because it's important to our health.  Most... to engage with friends, family, and the public about its importance.  I think many of us grew to assume...

  40. Engaging with science and society at pgEd

    Type
    Blog Post
    ...ways of collaborating, and to organize events that bring different voices together to talk about genetics...broadly with diverse communities, to raise awareness about where science is and where it is going, and to thoughtfully...currently work, strives to broaden the conversations about genetics and facilitate dialogue between scientists... In this post, I hope to tell you a little more about how I ended up at pgEd, what I do there, and how...similar position. How pgEd broadens the conversation about genetics pgEd, based at the Department of Genetics...of raising awareness and starting conversations about both the benefits as well as the personal, social...biomedical establishment. This, along with concerns about the use of DNA by law enforcement, continues to ...
Showing: 121 - 160 of 968 results