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We narrowed to 929 results for: KIN

Showing: 151 - 200 of 929 results

  1. Kit Free RNA Extraction

    Type
    Protocol
    ...or RNase AWAY®, may be used). For more tips on working with RNA, read this blog post on RNA extraction...well-ventilated space and under a fume hood when working with the volatile reagents in the list above. Procedure...freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of it and storing those in -80...

  2. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...2 Labs Learn how to best protect yourself when working in BSL-1 and BSL-2 labs Watch the Video! Lab Safety...Description (Link opens in a new window) Link to Video Making LB Agar Plates Create plates to culture bacteria...antibiotic to a pre-poured plate Watch the Video! Streaking Bacteria Isolate single bacterial colonies on ...

  3. General Transfection

    Type
    Protocol
    ...After 2 months, discard the tube and thaw a new working stock. Considerations Before You Start The health...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...DMEM complete. Incubate the cells 24–48 h before checking for protein expression. Sample Data Legend: Lenti-X...

  4. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Vortex ...Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells...

  5. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary...aspirate the wash buffer from the wells. Prepare the blocking buffer (1% BSA in PBS) as follows: Add 250 mg ...mix. Using a multichannel pipette, add 200 µL of blocking buffer to each well. Cover the plate with a plate...

  8. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...freezer and you are not 100% sure it is what you are looking for, but you have a map and know exactly what it...

  9. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Liquid Bacterial Culture You may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating...Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require...

  10. Centrifugation

    Type
    Protocol
    ...appropriate for the lab space in which you are working. Even if your samples may not require specific ...centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes into the rotor...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations... fine in an assembly if you want to save time. Working on ice, combine segments in the Gibson Assembly...

  12. Pipetting Protocol

    Type
    Protocol
    ...amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...

  13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL of 15% iodixanol...facing the 40% step. Collect up to 5 mL per tube, taking care to avoid collecting the proteinaceous material...

  14. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Wear a lab coat, eye protection and gloves when working with this chemical. Note: If you add EtBr to your...the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield, gloves...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

  16. CRISPR Library Amplification

    Type
    Protocol
    ... 30-37 ℃, 225 rpm for 1 hour. After the 1 hour shaking period, pool and gently mix the four tubes. Perform...transformation efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

  18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience working in a developing tropical country. This is why I set up a placement working in a rural...work-life balance compared to working in research. My previous experience working in Industry was really valuable...sustainable. After working at this company for several years, I was ready for a change.  Working abroad: Research...new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for... decide for themselves.  Here is my experience looking for the perfect job. Summer work experience in... explain why I changed jobs. I highly recommend taking on a similar position in your field of interest...and problem solving. I believe that is something lacking during degree courses; they never really prepare...

  20. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first...it, try making fresh running buffer. Old or improperly made running buffers can cause all kinds of odd ..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...pattern in your background, try asking more experienced labmates or looking it up online. You’ll find many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...

  21. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn.  Any time...how to get it, especially if introverted ), and networking (how to get people to remember you). What better...Watch other people give great talks We are not talking about lab meeting here. Lifeless presentation of... Series. Watch how these speakers talk without looking at their slides or notes. Listen to their stories...and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion...

  22. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...46 : How Kingdom ended up working in the life sciences and agriculture 5:47-9:35 : Kingdom's biotechnology...: Intro 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to...14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects...Seeding Labs is helping advance Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston...family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best...Biotechnology at Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata...research at Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University...

  23. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...presenting on building relationships (also known as “networking”), one of the most common questions I receive...starting to see the advantages of using social networking to start, build, and track professional relationships...professional contact information available. When I am speaking at a new institution, for example, I will often...d be much more likely to say  “Hey, thanks for looking me up on LinkedIn!” resulting in just a little ...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea.  Pro tip: ... unless you’re on a profile page. You might be looking at a list of suggested connections and you’ll see...complete strangers as it borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just...

  24. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...chat box and upvote If the thought of walking up to the mic and asking your question in a large conference...in case someone’s internet cut out. “ On networking Networking can be more difficult in the virtual format...organic networking,” she says.Depending on the meeting, there may be a “place” for networking within the...answered this way and the hurdle of getting up and asking in front of many people is lower,” says Bachle....,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question.”If the...in the chat box. That’s another benefit to note-taking: you can revisit the Q&A in the chat box. Presenting...the participants to learn about what they were looking to learn in the workshop so they could steer their...

  25. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...scientist who began working remotely recently? Most of us at Addgene began working from home last week... mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...week to flatten the curve, but working remotely can be more difficult if your work is based in the lab. ...sharing links to online yoga classes. Get outdoors. Taking a walk or a bike ride is a great activity for both...the desire and ability to, some communities are looking for bench scientists to assist in labs that are...volunteering to foster a pet, for example. If you are looking for some resources specific to self-care during...check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health. Work on personal...

  26. Antibodies 101: ChIP

    Type
    Blog Post
    ... to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working with a tissue...analysis process. And ChIP itself begins at...  Crosslinking Once you have your sample, the first step is...sample processing and therefore will require crosslinking, which uses formaldehyde or UV light to create...complexes together until you want to break them. Crosslinking is necessary for most ChIP experiences, but ...determine concentration.  Protein Capture After crosslinking and sonication or enzymatic digestion, you’re... not be a good choice) and the amount of cross-linking can affect epitope availability.  Collect the DNA...then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with a PCR purification...

  27. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...time for me to start considering my future path. Talking to Addgenies has helped me get a much better idea... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate school and...

  28. Lab to Office Culture Shock

    Type
    Blog Post
    ...there's a big office vs. lab culture shock). I'm looking to see if there are any resources that can help...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene.... What causes the lab → office culture shock? Working in an academic research lab involves a few key ...your family. I talked to a graduate student at a networking event recently who referred to her lab as her...applicant asked if she would partake in “critical thinking” in the position to which she was applying. I ... stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems...

  29. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where... cell culture, I would often respond to friends asking me to dinner saying something like, “I’ll be there...ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning out... this post useful. After a bit of research and thinking about my own processes, I’ve broken down time ...until it was too late. I didn’t spend enough time thinking about the possibilities and how to prioritize ...the day, month, or even the year, you should be thinking about two main things: How useful the task is...time it will take you to complete the task When picking which tasks to actually do, you should err on the...

  30. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...afford them). I am talking about making sure the audience is interested, educated and talking as a result of...moderator should have a well-planned approach to making the event interesting and engaging. Long before...the pre-event interviews, the moderator should be taking good notes and discovering interesting stories,... panelists have the correct date, location and parking information and remind them about the event as ... a really lively discussion, I strongly suggest asking the panelists not to use any slides for the event...prevent any one panelist or audience member from taking over. My own personal rule is that I do not let...chose good panelists, they are going to be good at talking, so they will easily take 5 minutes to introduce...

  31. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference. Networking All of the things we've mentioned above could be considered networking, but here ...tips. Before the conference Networking is key even if you are not looking for a job - those connections...the elevator pitch, talks, the poster session, networking, and social media. The elevator pitch Before...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...bored, or particularly excited about? If you tried tweaking your pitch throughout the conference, what worked...the poster session, send them a follow up email thanking them for their conversation and connect with them...social media. Social media Before the conference Networking can be made easier through social media. Use ...

  32. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...human kinases remains challenging. Small molecule kinase inhibitors usually target soluble kinase domains...Human kinase domain constructs for automated bacterial expression The misregulation of human kinases has...recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed...Threonine kinases while YopH (residues 164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion would...Arumugaswami lab at Cedars-Sinai Medical Center has kindly constructed and deposited an unpublished set of...

  33. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce that our plasmid and...GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive...identities of most features labeled directly on them making them much easier to quickly identify. Improved ...With this more functional display, you can start thinking about your next cloning experiment early on. Beyond...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...similar hover over features. As with the map tab, clicking on two separate locations in the linear sequence...

  34. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ... and then condensing that and making it accessible to students.” Making science approachable is often ...This involves going in front of a camera and kind of walking them through the steps of, for example, cutting...And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject matter expert Caitlin started out ...job as an SME: “I write curriculum for students taking science courses online, which is a blast. I have...an introduction that gives a basic overview of kinematics in physics, to developing a lab about mitosis...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also collaborates...

  35. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...basic one, making sure that you have a good fundamental understanding of what you’re working on is important...years. We each have a unique “origin story,” but taking the first step into the world of academic research...glassware with brightly colored hazardous liquids. Working in a lab is nothing like a typical part-time or... and applied. The current pandemic serves as a striking example of the applicability and importance of...be unsure, it helps to have a vague sense of what kind of research you would be excited about. I know quite...do what they do. I would also highly recommend talking to other undergraduate researchers, as well as ... yourself into is really important.  As you’re talking to people, though, try to be conscious of who has...

  36. The Challenges of Cell Culture

    Type
    Blog Post
    ...thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple... generally ensure cell line identity and purity making it easier for comparable studies to be performed...to check their identity. For example, perhaps a working cell bank has been made by a research institution... good recordkeeping, you need to have a way of tracking how many passages the cells have undergone and... any of the Master cell vials until all of the working vials have been used up. This ensures you can retain...means you can regularly discard the cells you are working with because you have plenty of maintained freezer...freezer stocks. It is vital that the Master and Working stocks of cell lines have the passage number recorded...

  37. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the...tools allow one to study the kinetics of cell migration and movement by marking cells at a specific time.... Pathogens - Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization...tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital microscopy. The video above...that some are more reliable and more efficient at tracking cells. Good two-photon probes when using a standard... cells and follicular dendritic cells within the skin and lymph node respectively. The permanent labelling...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     The past...

  38. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind of plate they...professionals from other backgrounds at conferences or networking events. And if you’re a wet lab scientist who...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...they were talking about during the meetings. And “plate” certainly wasn’t the only problematic word. How...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...creating shared experimental protocols. Clarify: Asking for clarification during a meeting can help get...

  39. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...release IL-36 cytokines. Keratinocytes are the main source of IL-36 cytokines in the skin making it important...the uncontrolled activation of the interleukin-36 (IL-36) cytokine family resulting in the activation ...disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...surrounding IL-36 biology. “Little is known how IL-36 cytokines actually get out of keratinocytes and induce their...researcher at University Hospital Zurich. IL-36 cytokines are a potent inducer of an autoinflammatory loop... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

  40. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... worth taking time to honestly evaluate your own style of work and communication before making this important...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might... toss data-driven reasoning out the window when making decisions. We think scientists make choices based...based on emotions and assumptions. I’m not just talking about simple things like “Which route should I ...members can give you a fuller picture. Consider talking to people in the lab across the hall or in the ...prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...t choose a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting...

  41. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...type of team can exist indefinitely. If you’re looking for this type of team in academia, look no further...an imaging core or tissue culture core, you are looking at a process-oriented team serving a particular...you find that there is not someone (or someones) taking on these responsibilities on a team to which you...is certainly necessary, but equally important is making sure all team members understand what roles they... none of that matters. If you are on a team, any kind of team, and you have nagging doubts that someone...

  42. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...of my job], so making it a success feels like a real accomplishment.” He started working on them shortly...not sure what his next step should be. He debated taking a position as an application support/sales representative... to me. She was leaving a writing position and looking for someone who might take over. I like writing... a science writer these days. I do some public speaking and a bit of video work for webinars (get tips...shortly after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars... writing group I am part of is responsible for checking the scientific accuracy of everything that goes...analysis.” Han’s biggest piece of advice for those looking to break into the field of science communications...

  43. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...back out when you are looking to change positions in the future. Anyone looking for work in today’s job...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...student or postdoc forever, and you need to be thinking about what comes next. To that end, one of the...for your career is to expose yourself to other working professionals, and one way to do this is to attend...and connections are how you get jobs! Job fairs Speaking of jobs, it seems like an obviously important ...conference, job fairs bring you face-to-face with working professionals, with the added benefit that most...larger meetings offering resources for students seeking employment within industry or academia; although...

  44. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where you...using creative problem-solving in the lab   Thinking Outside the Box: Individual Approaches Whether...something tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point...same problem. If your lab/company/team is still working towards a culture of encouraging dissent, the next...nine enemies that stand in the way of creative thinking. I won’t go through all nine here, but I’ll discuss...longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well received, understand...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

  45. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...cells. These cells produce inflammatory cytokines - interleukin (IL)-12 and interferon gamma (IFNγ) - and...help escape immuno-surveillance, and curb cytokine and chemokine production in the tumor microenvironment...their microenvironment by producing C-C motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive immune...tolerance beyond direct proximity to the tumor; chemokine-aided activation of APCs produces modified dendritic...cancer. Surgical removal causes increases in tumor cytokines like TGFβ and IL-6. However, when strengthened...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...response. Antibodies targeting the tumor-augmenting cytokines produced in the tumor microenvironment TGFβ, IL...

  46. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...with the end of year approaching. We’re always looking for something to brighten our day, whether it’s... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity, and photostability...pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs) are a major class of signaling receptors...spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. bioRxiv 2022.08.13.503850... al. 2022). iGECInano displays better response kinetics and photochemical characteristics than previous...

  47. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for...lead to hurt feelings, low morale, poor decision making, and stalled projects. You want to identify and...experiments to address a reviewer’s concerns, you are making your hypothesis stronger. When you share data at...miscommunications in the future. Assumptions: Making incorrect assumptions can be a direct cause or ...or consequence of lapsed communication. Making some assumptions about how something should be communicated...know that a person or team should be involved in making a decision and you see no record of their input... an eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion...

  48. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...addition, it’s really asking a lot of someone to be comfortable on camera while thinking about things like...Science Video Production Specialist, I’m constantly thinking about ways to improve our productions to deliver...we developed a great plan to start consistently knocking out high-quality videos for you all. The next ...products or methods, using metrics and feedback, taking a look at what works and what doesn’t, and trying...Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing Board Control Center...Center With the pandemic in full swing and everyone working from home, how were we going to film anything? ... prove to be difficult. Shipping the gear, and walking everyone through the recording process was going...

  49. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...wise to start working on these skills. Neuroscientists use lots of cool tools While walking around the exhibition...discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster presentation...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that almost... mathematical know-how for analysis. If you’re looking to get into neuroscience and you don’t have a background...neuroscience but are afraid you don’t have the right kind of biological expertise to get into the field, there...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

  50. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making available.... Addgene: Is this what you are working on now? Voigt: We are working on this now, we have ideas, but ...Massachusetts Institute of Technology has been “working on new experimental and theoretical methods to ...large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits become... been putting together very large toolboxes and making them available. We’ll also be submitting parts ...objective is to give people the complete toolbox for taking on very large genetic engineering projects. Addgene...you want. The last few years, we’ve really been making tons of parts and characterizing the software to...
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