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Showing: 61 - 80 of 435 results

  1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon Transform plasmid(s) into...Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S (2020) Multicopy...s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of CRISPR-Cas systems...doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...Gossen, M., Bujard, H., & Reed, S. I. (1994). Acceleration of the G1/S phase transition by expression ...transactivator (rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to doxycycline and three...tetracycline-responsive promoters . Proc Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas...Link opens in a new window) Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H....PMID: 8114703 (Link opens in a new window) Urlinger, S., Baron, U., Thellmann, M., Hasan, M. T., Bujard, ...expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (14), 7963–7968. https://doi.org/10.1073/pnas...

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...10.1038/s41589-020-0535-8 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled... 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream complexes involved...

  4. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss, S. N., Dernic, J., Bhat, M. ... Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li, Y... Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do you...

  6. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ..., C., Lian, C., Luo, S., Liu, L. D., Zhang, S., Zhang, Y., Wo, Y., Li, N., Wu, S., Gui, T., Wang, B., ....1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald...25217-y Zhao, D., Li, J., Li, S., Xin, X., Hu, M., Price, M. A., Rosser, S. J., Bi, C., & Zhang, X. (2021...Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:G to G:C...Replogle, J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti...split vector approaches developed in the early 2000’s which allow researchers to deliver oversized cargo...and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression in the K562 cell line (Bak et al....2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess, S. J., Ferling, I., Gazda, M. A., Hoeppner...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E....Prepare and label a sample that expresses your target(s) at a high level, either naturally or by overexpression... D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant...10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction...

  9. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...-7_20  Karmakar, S., Panda, D., Panda, S., Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, ...  References and Resources References Karmakar, S., Panda, D., Baig, M. J., & Molla, K. (2025). A unified...10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021). Precise plant genome...s41477-021-00991-1  Qiao, J., Zang, Y., Gao, Q., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & ...

  10. Plan Your Experiment

    Type
    Collection
    ...designed your gRNA(s), the next step is to choose how to express your Cas enzyme and gRNA(s) in your target...., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J. S., Friedman, N., & Regev, A. (2016). Perturb-Seq...Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann, M., & Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated...References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system that...generally only compatible with smaller Cas enzymes, like S. aureus Cas9. For more information, see our blog post...

  11. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography,...Institute and MIT published another report showing Cas9's structural details. "Understanding this structure ...This Protein Could Change Biotech Forever."  David S. Weiss of Emory University summed it up well in that...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren.... References and Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A.....67.8.3440-3444.2001 Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R....2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications...

  13. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Western Ontario. Adam summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional... the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous...potential of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to test if the exogenous...technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 ...Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature...2013 Jan; 493, 429–432. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

  15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...and resources References Dong D, Guo M, Wang S, Zhu Y, Wang S, Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural...Inhibit DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017),...deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. Phage therapy Phage...doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/...

  16. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019) Gene activation by dCas9-CBP and...

  17. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ..., C., Baier, A. S., Pascolutti, R., Wegrecki, M., Zheng, S., Ong, J. X., Erlandson, S. C., Hilger, D.,...., Rasmussen, S. G. F., Ring, A. M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display platform...https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu, D. R. (2015). Methods for the directed evolution...nrg3927 Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021). Directed Evolution: Methodologies...://doi.org/10.1021/acs.chemrev.1c00260 Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR...

  18. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...

  19. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...10.1038/nbt.3947 Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998)....gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are ..., R., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...
Showing: 61 - 80 of 435 results