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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 347 results for: ATC

Showing: 61 - 80 of 347 results

  1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...necessarily expecting a perfect match - we will often find a few mismatches in the origin of replication ...sequencing, we feel confident that these few minor mismatches usually don’t affect the function of the plasmid.... We also confirm that the species of the gene matches the data associated with the plasmid.     Confirming...

  2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ..., the Roska lab built synthetic promoters from scratch and tested to see if they drove cell type specific...channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing ...Synthetic promoters did, however, tend to loosely match the specificity of their source genes, i.e. if a...drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result...

  3. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...scientific poster apart? First, a good poster should catch the audience’s eye and draw people in. That can ...may choose to display it as rows. It can be more catchy because it is generally less common and will stand...advantage of being different and potentially more catchy, but it can be trickier to make figures fit nicely...relevant to the work you are presenting. The latter is catchy, but beware. A distracting background or clashing...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...interest. Every batch of AAV requires its own MOI optimization to account for batch-to-batch variability....Ultracentrifugation Protocol for more details. Pro tips Watch this AAV purification video! It didn’t exist when...

  5. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...any discrepancies in Sanger sequences, such as mismatches, deletions, or insertions. We use blastp or blastx... the species that your sequencing result should match, enter the common or scientific name into the Organism...smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you are curious...Sanger sequencing result and noted any potential mismatches or gaps in the nucleotide sequence, you can run...

  6. Genetic Code Expansion

    Type
    Collection
    ...a previously established protocol, make sure to match the growth medium, ncAA concentration, and the cell...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...NatureJobs: Interviews: The All-Important Job Talk. 1. Watch other people give great talks We are not talking...are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking at their...entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor...of saying “so” and “um” a lot. I hate it when I watch myself speak, but others tell me that I keep the...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers to successfully...protocol. Be sure to adjust the extension time to match the size of your template. Amplification of GC-rich...only DNA that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR ...transform your PCR products into will efficiently patch up the DNA. The resistance marker from the parental...

  9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...fragments. As a fun way to start, I highly recommend watching this entertaining video created by our friends...want next to it, so this doesn’t allow for mix and match of many different fragments. One way around this...homology regions, which allows for the mixing and matching of parts. Gibson assembly meets CRISPR Gibson... Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene ...

  10. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do a negative...the number of colonies obtained using your fresh batch of competent cells with those obtained using commercially...commercially available cells or previous batches of homemade competent cells to ensure good efficiency....

  11. Deep Dive: qPCR

    Type
    Blog Post
    ...optimized until the multiplexed and individual values match across the board. If the samples or sample conditions...need to make sure the assumptions in your dataset match the assumptions in your analysis method. ΔΔCT/CQ...’re not sure if your amplification efficiencies match, you’ll need to run a validation assay in which ...favorite (...er, whichever one uses assumptions that match your data set, that is.) Yuan, 2006 has a good overview...

  12. Antibody Guide

    Type
    Collection
    ...small aliquots of many different antibodies and do batch validation up front rather than risk delaying a ...

  14. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...team in the UK is five hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation... team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications with my UK team members...out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when I ...

  15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...networker (or if you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships... the country with groups who are piloting this. Watch for my upcoming mentoring post in which I describe...naturally out of friendships or work interactions. Watch for the people that are already teaching you and...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    ...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences...

  18. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... “copies.” This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies,...to non-target proteins), variability (separate batches can perform differently – happens when the antibody...

  20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up...genetic cross). You can tell when pupae are ready to hatch the next day because the pupae will develop a visible...
Showing: 61 - 80 of 347 results