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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 638 results for: Erf

Showing: 61 - 80 of 638 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... follow the kit's instructions. If you want to perform plasmid purification without using a kit, you can...ribonuclease that digests single-stranded RNA. Optional: Perform phenol-chloroform extraction - see protocol below... DNA purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant...

  2. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). Basic PCR Program...pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: Continued...reagent by the number of individual PCRs you wish to perform and add 10% extra to account for pipetting error...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... of a stable cell culture after transduction. Performing antibiotic selection on transduced cells enables...5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells...untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  5. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  6. Serotype Testing AAV

    Type
    Collection
    ...economical choice that can be used to validate the performance of various serotypes before conducting larger...

  8. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

  9. AAV for Neuronal Tracing

    Type
    Collection
    ... AAV can be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...form of scFvs A simple vector system to improve performance and utilisation of recombinant antibodies. BMC...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...with Transient Plant Leaf Transformations RNA Interference in Plant Biology: New Tools for an Old Favorite...

  15. Lentiviral Prep Service

    Type
    Collection
    ...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

  17. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  19. COVID-19 Resources

    Type
    Collection
    ...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...
Showing: 61 - 80 of 638 results