Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 343 results for: GAI

Showing: 61 - 80 of 343 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...50% per freeze thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the virus will be used in a couple...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...PMID: 10325307. PubMed Central PMCID: PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral...

  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and to repurpose...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...spreads through the wild population and immunizes it against the first drive. Laboratories interested in building...question), why not use them whenever applicable?Against this, consider the cost of an accidental release...accidental release could delay real-world applications against scourges such as malaria and dengue for many years...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    ...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes...Good for purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA...  Combo See text  TRX MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL...

  5. Lab to Office Culture Shock

    Type
    Blog Post
    ...gut-wrenching, but you can get that feeling of camaraderie again in a non-academic career. You might not be in a ...you still find the idea of not holding a pipette again upsetting, there are ways to get back to the bench...opportunities. You can get your hands in nitrile gloves again! The opportunities are there once you start looking...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy...for precise correction of Rett syndrome mutations. Gail Mandel and her team have shown for the first time...being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous technical challenges ...

  8. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...every experiment, an investigator needs a standard against which the results can be compared; results from...single experiment wherein Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected into...included as these provide the baseline/standard against which other samples can be compared. The Empty ...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...secondary antibody that recognizes the primary, wash again, then image. But here’s where the “sequential” part...add the new primary, wash, add secondary, image again, inactivate, and repeat these cycles for all your...yourself. If you can’t find a suitable antibody against your target protein, consider fusing a protein ...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...instead of the population as a whole, scientists gain statistical power in their observations. However...receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer. ...methods. In direct staining, the primary antibody against a target is conjugated to a fluorochrome. In indirect...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...approach, which focuses on validating antibodies against markers of the human blood leukocyte populations...on human blood leukocytes, including antibodies against these markers. The 371 CD markers characterized..., and include information on tested antibodies against the target. Helpfully, this also includes clone...

  12. Adenovirus Guide

    Type
    Guide
    ... used viral vector platform for vaccine design against a diversity of viruses. This guide contains many...this, and to the strong host’s immune response against the transduced cells, transgene expression delivered...mammalian hosts and have been used as vaccines against infectious diseases such as COVID-19, Ebola virus... immunity to Ad5 and protect nonhuman primates against ebolavirus challenge . Journal of Virology, 85 ...

  13. Molecular Biology Reference

    Type
    Guide
    ... within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Additionally...out-populate bacteria with plasmids, thus selecting against the propagation of these plasmids through cell ...These attached DNA templates are then amplified again producing ~1,000 copies of each template. Each template...color that is emitted each time a base is added. Again, each base (A,C,T, or G) is labelled with a different...

  14. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...impressive science-themed holiday decorations yet again. This year, we had two different categories: one...his Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This ...

  15. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...special note as the open science movement continues to gain traction. One way to consider where the open access...Court’s decision to ban gene patents in a case against Myriad, an organization called Free the Data launched...

  16. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...access your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming...labmate? Though electronic lab notebooks (ELNs) have gained traction with pharmaceutical companies, academic...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit mission...enabling more scientists in labs all over the world to gain access to new materials and information. Core values...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...collection provides scientists with a toolkit to gain an understanding of the signaling through the transducerome...facilitating screening across the transducerome, to gain a more complete understanding of a GPCR mediated...

  19. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... antibodies drive an effective immune response against a pathogen. The Fc domain creates a link between...Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi...

  20. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...
Showing: 61 - 80 of 343 results