...PRLTS PDYN prodynorphin MGC26418, PENKB PENK proenkephalin - PF4 platelet factor 4 CXCL4, MGC138298, SCYB4... Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External Resouces Immport : Bhattacharya...

...Talk." 4. The chalk talk, simple in principle The first question I usually get about chalk talks is, what... whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk or markers force ... Developing your Chalk Talk These items should be part of the beginning of your chalk talk. These should... This edition has substantial expansions on the chalk talks and Zoom interviews sections, along with new...what is a Chalk Talk? It is a 60-90 min session with department members to discuss your research plans...alternatives for an approach, if asked. The key to a chalk talk is to effectively convey your message. Key ...." This is only the first few minutes of the chalk talk. Realize that it is rare to make it through...

... making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy at ...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, work...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely...absolutely equally, which means you only have one-half of the guilt. You can handle that, right? Big tip...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...

...Nabet et al., Chemical Biology, 2018.    HaloTag The HaloTag was initially developed as an affinity tag...the Halo-tagged protein in question, it is targeted to the proteasome for degradation. The HaloTag is ...straightforward system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road. It is difficult...

...bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical...Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology 233.39 (1971): 149...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found... excitation, whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus 2/9 delivery of...

...while writing each section. Research challenge The Research Challenge motivates the request for application...how you, the expert, will address the research challenge. Now is the time to make bold statements. While...solution is the best approach to the research challenge. Demonstrating expertise in the field and past...how your innovation will address a variety of challenges and impact the field - its encouraged to dream...time budget writer, you may find this section challenging because it’s hard to determine the exact value... differs from publication writing and may be challenging to master at first. Additionally, reach out to...

...funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly politicized...., “The Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other...ramifications. Readers unfamiliar with academia’s challenges with publication and funding may be dismayed ...The three chapters in this section describe the challenges of trusting science in a “fake news and post-...describes the author’s thoughts on science’s biggest challenges over the coming years. It is arguably the most...the book? It doesn’t need to. The problems and challenges described in The State of Science have not changed...may be the introduction they need to grasp the challenges you face in your career.  Acknowledgements: ...

...the power of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...co-selection approach relying on repair of a conditional-lethal mutation to identify edits with minimal screening...screening effort. Selecting for repair of a lethal mutation should remove much of the unedited “haystack”, ...viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...Engineering of theCaenorhabditis elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ...

...for adding the next nucleotide, the reaction is halted once the fluorescently tagged base is incorporated...converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does not require...

... N713) and C-terminal (residues 714-1368, C714) halves, rendering the Cas9 non-functional when split but... but regaining functionality when the halves are reassociated. By fusing a photoinducible, heterodimerizing...saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly ...Nihongaki et al., 2015   CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Polstein...Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Nihongaki... al., 2017 Split-CPTS2.0 CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Kim et... et al., 2019 LADL CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription. Label genomic...

...packaging capacity is limited to ~4.7kb, or roughly half the packaging limits of lentiviral and adenoviral...multiple copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440...heterodimer, then splicing of the pre-mRNA of the two halves of the transgene will reconstitute the full-length...A of the transgene contains the promoter, the 5’ half of the transgene, a splice donor sequence and the... of homology, a splice acceptor sequence, the 3’ half of the transgene. The full length gene is reconstituted...124–130 . https://doi.org/10.1038/sj.mt.6300322 Halbert CL, Allen JM, Miller AD (2002) Efficient mouse ...

... Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying the abundance... in vitro against different drugs and dosages.  Michal Bernach: Developing an artificial leaf replica ...plant-bacteria and bacteria-bacteria interactions  Michal Bernach is a graduate student in Mitja Remus-Emsermann...surfaces. His system of choice is the Arabidopsis thaliana - Pantoea agglomerans interaction. But the shape...bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach plans to use a suite of fluorescent...

...algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary...to making cancer precision medicine a reality. Challenges for cancer variant characterization For many ...validation experiments. But, there have been two huge challenges preventing us from testing this hypothesis: ...Accelerator Plasmid Collections To overcome this first challenge, we’ve developed the Broad Institute Target Accelerator...cancer variants To overcome the second major challenge, we developed assays to test large numbers of ...

...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications..., is composed of only 529 amino acids, less than half the size of either Cas9 or Cas12a. Despite its small...

...optogenetics.  Challenges of engineering channelrhodopsins There are three main challenges to engineering...overcome channelrhodopsin design challenges To overcome these challenges, the Arnold and Gradinaru Labs ...lower multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because it’s lower...

...since the Future of Research Symposia Ongoing challenges facing the scientific enterprise and the way ...landscape of the scientific enterprise will provide challenges and opportunities for us all.  For example, implementation...categorize all postdocs at institutions, will be challenging. At the same time, there is much opportunity ...the pressure on for further increases will be challenging, but will be aided by the development and implementation... is an important way to push the balance from challenge to opportunity. We hope to see you at the upcoming...

...proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems, in contrast to single-protein...with the transposition protein TniQ for targeting (Halpin-Healy et al., 2020). Genomic targets require an...creates a 5-bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons...follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS...doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH (2019) Transposon-encoded...

...DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this lethality to design...organisms, CRISPR-induced double stranded breaks are lethal because the non-homologous end-joining (NHEJ) repair...Cas9 will cleave the gene of interest, inducing a lethal DSB.     This system is distinct from those used...While the activation of transcription is a more challenging problem than repression, scientists have deposited...Streptomyces, yet genome engineering remains challenging for most organisms in the genus. Work from the...

...solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies... Sharing your research with the world can be challenging. After months, or years, of grueling effort to...biology or international relations.  Many of the challenges in science today are too complex for any single...making publishing their research all the more challenging even when it is of quality). On the site you ...

...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record...local proteome, but some applications have been challenging or inaccessible. The Ting lab recently made two.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...