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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 185 results for: INV-E

Showing: 61 - 80 of 185 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...and strategies for their projects. References 1. L. E. Woodard and M. H. Wilson, “piggyBac-ing models and...Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J.... Menon, G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

  2. CRISPR in the Clinic

    Type
    Blog Post
    ...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many...Hussein M, Molina MA, Berkhout B, Herrera-Carrillo E. A CRISPR-Cas Cure for HIV/AIDS. Int J Mol Sci. 2023...strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived...returned to the patient. Finally, allogenic therapies involve editing healthy donor-derived cells and delivering... treatment strategies for these disorders have involved turning on fetal hemoglobin to compensate for ...returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was sponsored by...evolving, and many of the challenges with therapies involve patient safety, effectiveness, and delivery. Patient...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...345, 626–628 (2014). PubMed. Gantz, V. M. & Bier, E. The mutagenic chain reaction: A method for converting... doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church...damage public trust in scientists, experiments involving potential gene drives should be conducted with...strategies are available? Molecular confinement involves building gene drives that can spread through populations... been tested in yeast5. Ecological confinement involves performing experiments in a geographic area where...relevant mosquito species. Reproductive confinement involves working with laboratory organisms that can't reproduce...publications. Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin ...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... In the late nineties, this was developed into an E. coli system, with the noncanonical amino acid in ...expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG codon for...components, meaning that the final protocol may involve a sensitive balance of trade-offs. This balance...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...carries CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) What genetic modification... are identified using next-generation sequencing (E). Considerations and tips for successful screens... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...a population of mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily...specific classes of human genes. What are the steps involved in a CRISPR screen? Performing a forward genetic...

  6. The 12 Days of CRISPR

    Type
    Blog Post
    ...On the 11th day of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene... the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function using transcriptional...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the...for now-common business practices, like accepting e-signatures and designing a website users could easily...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

  9. CRISPR Library Amplification

    Type
    Protocol
    ...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if...pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use caution when...

  10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

  11. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic...team also investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...

  12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification...Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...us your plasmid information in spreadsheet format, e-mail us at  [email protected] Special cases Please...can distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were...allow you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector....deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...

  14. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ... UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter...Operating Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology...

  15. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...to cancer.   References and resources Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M.,...pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to cancer. The screen...

  16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Thornhill, S. I., Parsley, K. L., Staal, F. J., Gale, R. E., Linch, D. C., Bayford, J., Brown, L., Quaye, M.,...Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type 1 integrase... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...wish you the best of luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of..., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D....sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ....1007/978-1-0716-2918-5_5 Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard, K., Cepko, C.,....2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson, L. E., & Fan, M. (2016). Practical Considerations for Using...This process builds on our former approach and involves reading primary literature, soliciting information...

  20. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first time. After...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... in learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and...
Showing: 61 - 80 of 185 results