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We narrowed to 526 results for: MEA;

Showing: 61 - 80 of 526 results

  1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...is a measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...Specificity is the measure of off-target activity (left) while sensitivity is the measure of on-target activity...sensitivity. Site accessibility Site accessibility is a measure of which genomic sequences can be targeted by the...frequency at a target site. A specificity ratio can be measured by calculating the on-target activity divided ...

  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...trouble if you don’t qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind...official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS tracking system....collaborators will not have the context you do, which means you’ll need to provide that context yourself. Simple...corporate counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager... meeting is using the same words with the same meanings consistently, meetings will be shorter and more...productive. Higher Productivity. Taking active measures in improving cross team communication has helped...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration...ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration of viral particles...Lock et al., 2010). It is recommended that you measure your infectious titer, since it will have more ...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...your isotype.  Antibody concentration may also be measured through a protein stain, such as Coomassie blue...Though more time-consuming, this assay will also measure the purity of your prep. In this method, antibodies...standard. The density of the antibody bands can be measured against the protein standard and the concentration... beside the heavy and light chains can also be measured. Contaminating bands lower the purity of your ...CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of...monoclonal antibodies with experimental values as measured by the Edelhoch method. Int J Biol Macromol. 2015...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...that the results can vary by a factor of 2. This means that if you set up two identical assays with the...ddPCR) does not require a standard or a reference, meaning saving on reagents (and time)! AAV titering using...reader extracts the droplets from the plate and measures the fluorescence amplitude of each one. Droplets...outputs an image of the fluorescence amplitude measured for each droplet in each well (Figure 2). A clean...detecting fluorescence so that it is possible to measure the concentration of multiple targets simultaneously...detection of microbes. Some applications include measuring viable probiotics and detecting circulating pathogens...

  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...our Transferable Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution...be willing to face conflict head-on. That doesn’t mean you have to like conflict. Very few people do, and...all costs or actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable... of conflict resolution. Having empathy does not mean you like everyone you meet. Most people can’t maintain... of positivity past the age of five. But it does mean that even if you find someone annoying or disagreeable...party might get exactly what they want, but that means the other party will feel punished or invalidated...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...binding sites per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel...Reticulum (OSER) assay (Costantini et al., 2012) measures homodimerization of FPs targeted to the endoplasmid...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected...,” but I find this very confusing because it can mean that the fluorescent protein is at the C-terminus..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3..."A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in...

  8. Technical Design of a Western Blot

    Type
    Blog Post
    ...primary antibodies as needed. However, more antibodies mean more optimization and likely higher background noise.... A wet transfer is the most efficient method, meaning that it will transfer the most protein from your...light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein in the femtogram...sensitive but has a limited dynamic range, which means it may take multiple exposures for a blot with varying...you can perform relative quantification, which measures protein amounts and compares the amount of one...use total protein loading. Total protein loading measures overall protein expression and does not rely on...protein expression. Total protein expression can be measured using a protein stain like Coomassie Blue or Ponceau...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive validation...because an antibody can bind to its target, does not mean that is all it will bind to - particularly when ...performs as expected in one application does not mean that it will perform as expected in another. You...like all of science, validation experiments don’t mean anything unless you share the results with others...consider sharing your validation attempts by other means, such as through antibody databases like Antibodypedia...antibody doesn’t work in your application doesn’t mean it’s a bad antibody but more that it’s misunderstood...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...? The TRUPATH collection allows researchers to measure the physical association of combinations of different... signaling pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance...version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence...decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio of GFP2 emission...both antagonists and inverse-agonists could be measured using the TRUPATH system. Conclusions Although...

  11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ... described, then measured electrophysiological potential afterwards. This measurement trended with expression...stimulate or manipulate responses through neurological means like electric stimulation, drug administration, ...fibers to emit light for optogenetic purposes, measure temperature, study electrophysiology, and deliver...Alternatively, the system could be used to deliver or measure new variables. The sky is really the limit for ...

  12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by...ApoB bound lipoproteins (ApoB-LPs) are indirectly measured through their triglyceride or cholesterol contents...correlation with cardiovascular disease and indirect measurement of ApoB-LPs through triglyceride/cholesterol ...al, 2012; Lawler et al, 2017).  In an effort to measure ApoB-LPs directly and develop a potentially high... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...proteins are out of the measurable range of the dilution used in an assay, meaning that your normalization...you see a smiley face in your gel, or if you see smeared bands, your voltage is likely too high. If you’...   Ladders If you need very accurate protein measurements, consider using an unstained protein ladder,...conjugated antibody. If you do not need such precise measurements, you can use a colorimetric ladder, visible ...to adjust ‌your antibody dilution to accurately measure these proteins. That being said, normalizing to...dilutions are showing the same signal (Figure 3B), that means the amount of protein loaded is outside the detectable...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...(usually a laser in fluorescence assays), which means luminescence assays have lower background levels...luciferase gene, and expression of luciferase is measured via the light produced. A similar approach can...assays require cell lysis as the most efficient means to disrupt the cell membrane and deliver the substrate...luciferase or alternative substrates can permit measurements of luminescence from live cells. Luciferase ...with experiments that supply both substrates and measure luciferase output at different wavelengths. Addgene...

  15. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... this class measures either factors as proxies for a cellular process or directly measure a cellular process...boiled down pretty simply – they are systems that measure biological phenomena by generating an output which...microscope, plate reader, or any other instrument which measured GFP levels and converted that signal into a readable...high tension. These systems are powerful tools to measure real-time cellular reactions to stimuli and as ...

  16. Antibody Guide

    Type
    Guide
    ... two types of antigen specificity: monoclonal, meaning many copies of one clone that are specific to a... a certain epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes...bound. Signaling molecules , proteins that emit a measurable light or color in response to a specific stimulus...proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here, HRP is used as ...samples can be normalized to a total protein measurement. Both methods will also serve as a control for...conjugate, typically HRP, is activated and its output measured. This method can be performed using the indirect...Fix your samples. Prepare your samples using permeabilization, antigen retrieval, and/or sectioning. Block...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...fixation and permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol.... can also be affected by cell fixation and permeabilization techniques — and so it may be necessary to...mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells were stained ...also save money!); and optimizing fixation/permeabilization reagents for intracellular targets. Since...sample with mixed expression, with the expression measurable through an epitope tag such as FLAG/His/GFP. ...

  18. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... antibodies may have similar emission spectra, meaning they will emit fluorescent light at similar wavelengths...record the fluorescence for both at similar levels, meaning that your populations will ‘look’ similar on your...two channels (Figure 1A). Applying compensation means you are telling your cytometer to adjust the fluorescent... does NOT change anything in your samples. This means that, unlike voltages, you can adjust compensation...compensate in the top right corner of a flow plot, meaning that it should be double-positive for the two markers...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...between 0.80-1.20) would mean you have one copy of the gene; an RQ value of 2 means you have two copies, ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re... a light technical control - an RQ value of 1.5 means you have either made a pipetting error or an incredible...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ...commonly used to measure relative quantification of mRNA, which can be used as a proxy measure for differences...primers. This separate thermocycle allows you to measure the intensity of signal over an entire cycle. Since...want to validate your primer/probe through another means, such as an agarose gel or uMelt analysis (Downey...verification. Primer dimers will often show up as a smear or band around 30-50 bp. uMelt analysis  uMelt is...previously, just because it’s used often doesn’t mean it’s right for your experiment! The model assumes...
Showing: 61 - 80 of 526 results