We narrowed to 527 results for: MEA;

...Viral Preps from Addgene Measuring the pH of our samples: What we did To measure the pH of our samples, ...few experiments to determine if our tubes were permeable to the CO2 released from dry ice, and whether ... is that some of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in...the color of the liquid solution and pH paper to measure the pH. We compared the dry ice-stored samples ...Not all tubes (or rather, not all O-rings) were permeable to CO2 at low temperatures. We tested two different...tubes after the indicated incubation periods, and measured the pH. We found that tubes subjected to a recovery...

...give? Ask yourself if the results mean what the authors say they mean. Could there be any other possible...substantiate the facts upon which it builds. This means you have to become familiar with a body of literature...Finding reliable references and sorting out what they mean is no small task. As a scientist, it is useful to...the work, the research that was done and what it means. The introduction should give you just enough information...experiments, what data they collected and what it means. There should be some connection or flow among the...validating work prior to publication, it doesn’t mean that there are no mistakes or misinterpretations...

...the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you expect it to produce... the advantage of being significantly smaller, meaning they can recognize epitopes that full antibodies...can be used for qualitative and quantitative measurement of protein expression in cell lysates, whole ...cultured cells. ELISA can be used to quantitatively measure the amount of protein present. The reaction takes... (OD) - of the known and experimental wells is measured. To find the exact amount of protein in your sample...subsets of interest from among the mixture and to measure the relative amount of your protein of interest...the cell surface, or the cell membrane can be permeabilized to allow antibodies to bind to intracellular...

...group and C, technical replicates or repeated measurements will increase the power of the results. In this...experiment. Technical replicates are repeated measurements of the same sample and are included to assess...the process could make it difficult to obtain meaningful results.  Biological replicates are distinct ...with ‌secondary antibody as usual and the signal measured. If a nonspecific signal is detected, the level...to include a loading control. A loading control measures the protein level of a target that is thought ... the same membrane that the target protein was measured on.    Flow cytometry In addition to the controls...controls for antibody-based experiments, it is by no means an exhaustive list and we encourage you to review...

...Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring DA and other...technique is performed with a probe surrounded by a semipermeable membrane, which is inserted into the region ...causes the passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...powerful new developments in the detection and measurement of neurotransmitters in various species and while...

...drop onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the safranin...a species of bacteria, but what does it actually mean? What relevance does it have on the structure of...pretty simple process. First, flood the heat-fixed smear with the crystal violet stain for 1 minute. Rinse...procedural instructions, including timing, preparing the smear, and mixing the stains and solutions used, check...since gram negative and positive bacteria are permeable to different classes of antibiotics. The classification...

...is a measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...Specificity is the measure of off-target activity (left) while sensitivity is the measure of on-target activity...sensitivity. Site accessibility Site accessibility is a measure of which genomic sequences can be targeted by the...frequency at a target site. A specificity ratio can be measured by calculating the on-target activity divided ...

...trouble if you don’t qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind...official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS tracking system....collaborators will not have the context you do, which means you’ll need to provide that context yourself. Simple...corporate counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager... meeting is using the same words with the same meanings consistently, meetings will be shorter and more...productive. Higher Productivity. Taking active measures in improving cross team communication has helped...

...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration...ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration of viral particles...Lock et al., 2010). It is recommended that you measure your infectious titer, since it will have more ...

...that the results can vary by a factor of 2. This means that if you set up two identical assays with the...ddPCR) does not require a standard or a reference, meaning saving on reagents (and time)! AAV titering using...reader extracts the droplets from the plate and measures the fluorescence amplitude of each one. Droplets...outputs an image of the fluorescence amplitude measured for each droplet in each well (Figure 2). A clean...detecting fluorescence so that it is possible to measure the concentration of multiple targets simultaneously...detection of microbes. Some applications include measuring viable probiotics and detecting circulating pathogens...

...your isotype.  Antibody concentration may also be measured through a protein stain, such as Coomassie blue...Though more time-consuming, this assay will also measure the purity of your prep. In this method, antibodies...standard. The density of the antibody bands can be measured against the protein standard and the concentration... beside the heavy and light chains can also be measured. Contaminating bands lower the purity of your ...CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of...monoclonal antibodies with experimental values as measured by the Edelhoch method. Int J Biol Macromol. 2015...

...our Transferable Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution...be willing to face conflict head-on. That doesn’t mean you have to like conflict. Very few people do, and...all costs or actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable... of conflict resolution. Having empathy does not mean you like everyone you meet. Most people can’t maintain... of positivity past the age of five. But it does mean that even if you find someone annoying or disagreeable...party might get exactly what they want, but that means the other party will feel punished or invalidated...

...binding sites per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel...Reticulum (OSER) assay (Costantini et al., 2012) measures homodimerization of FPs targeted to the endoplasmid...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected...,” but I find this very confusing because it can mean that the fluorescent protein is at the C-terminus..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3..."A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in...

...primary antibodies as needed. However, more antibodies mean more optimization and likely higher background noise.... A wet transfer is the most efficient method, meaning that it will transfer the most protein from your...light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein in the femtogram...sensitive but has a limited dynamic range, which means it may take multiple exposures for a blot with varying...you can perform relative quantification, which measures protein amounts and compares the amount of one...use total protein loading. Total protein loading measures overall protein expression and does not rely on...protein expression. Total protein expression can be measured using a protein stain like Coomassie Blue or Ponceau...

...validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive validation...because an antibody can bind to its target, does not mean that is all it will bind to - particularly when ...performs as expected in one application does not mean that it will perform as expected in another. You...like all of science, validation experiments don’t mean anything unless you share the results with others...consider sharing your validation attempts by other means, such as through antibody databases like Antibodypedia...antibody doesn’t work in your application doesn’t mean it’s a bad antibody but more that it’s misunderstood...

...? The TRUPATH collection allows researchers to measure the physical association of combinations of different... signaling pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance...version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence...decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio of GFP2 emission...both antagonists and inverse-agonists could be measured using the TRUPATH system. Conclusions Although...

... described, then measured electrophysiological potential afterwards. This measurement trended with expression...stimulate or manipulate responses through neurological means like electric stimulation, drug administration, ...fibers to emit light for optogenetic purposes, measure temperature, study electrophysiology, and deliver...Alternatively, the system could be used to deliver or measure new variables. The sky is really the limit for ...

...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by...ApoB bound lipoproteins (ApoB-LPs) are indirectly measured through their triglyceride or cholesterol contents...correlation with cardiovascular disease and indirect measurement of ApoB-LPs through triglyceride/cholesterol ...al, 2012; Lawler et al, 2017).  In an effort to measure ApoB-LPs directly and develop a potentially high... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

...proteins are out of the measurable range of the dilution used in an assay, meaning that your normalization...you see a smiley face in your gel, or if you see smeared bands, your voltage is likely too high. If you’...   Ladders If you need very accurate protein measurements, consider using an unstained protein ladder,...conjugated antibody. If you do not need such precise measurements, you can use a colorimetric ladder, visible ...to adjust ‌your antibody dilution to accurately measure these proteins. That being said, normalizing to...dilutions are showing the same signal (Figure 3B), that means the amount of protein loaded is outside the detectable...

... this class measures either factors as proxies for a cellular process or directly measure a cellular process...boiled down pretty simply – they are systems that measure biological phenomena by generating an output which...microscope, plate reader, or any other instrument which measured GFP levels and converted that signal into a readable...high tension. These systems are powerful tools to measure real-time cellular reactions to stimuli and as ...