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We narrowed to 261 results for: Mac

Showing: 61 - 80 of 261 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder and CEO of Research Space, which...in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional resources on the Addgene blog...

  3. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...orchestrating blood-vessel formation. Precise, real-time pharmacology of this target has been hampered by transient.... G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. B., Benink, H. A., Eggers, C. ...complexes in living cells. British Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...temporal control than is achieved by traditional pharmacologic methods. A number of strategies have been developed...where to activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can...doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research, 93, 144...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...simplicity, other promoter elements and transcription machinery are not shown. Tetracycline Off (Tet-Off) The ...

  14. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

  15. Science as Art

    Type
    Blog Post
    ...non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a ...teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus revealing the...

  17. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

  18. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...sgRNAs, but they may or may not include CRISPR machinery (which can be supplied separately). Libraries ...highlight a prokaryotic library: the Cultivarium MACKEREL Library, whose acronym means Modular, NGS-trACKable...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series...Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis...
Showing: 61 - 80 of 261 results