...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...

...temporal control than is achieved by traditional pharmacologic methods. A number of strategies have been developed...where to activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can...doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research, 93, 144...

...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...

...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and...

...Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. 2015. CETCh-seq: ...

...clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter. Primer Validation...

...repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids...

...were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),...

... enhancers were derived from human, mouse, and macaque genomes. Enhancers were evaluated in mouse brains...

...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis...

...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

...non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a ...teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus revealing the...

...CMV Parkinsonism Pietro De Camilli 187767 MAC_C_CSF1R CSF1R MAC Hereditary diffuse leukoencephalopathy Markku... GFP U6, Cbh Spinocerebellar ataxia 6 Harold MacGillavry 131791 pHAGE-N-FLAG-HA-TBK1 TBK1 Flag, HA CMV...

...Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course, like...

...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series...Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis...

... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...