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We narrowed to 237 results for: Mammalian expression plasmids

Showing: 61 - 80 of 237 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post
    ....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...networks Double inverted vectors expressing light inducible ion channels (plasmid # 20298 for example) have ...all labeling plasmids and the low concentration of recombinase restricts their expression to individual...specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow...means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about...optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular...SV40 Large T nuclear localization sequence) and expressing it from a CAGGs promoter. However FLPe is still...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...curated collections of plasmids as kits with greatly reduced costs per plasmid. We generally create kits...: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq PUF Assembly...of promoters to facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus...just the tool you need. The plasmids in this portion of the BIOFAB Plasmid Set contain terminators of ...your polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the...Process with pCri Cell-free protein expression kit Cell-free expression systems have been improved for both...comparison of different cell-free expression systems; the Cell-free expression test kit represents a set of...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ... of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID along with your...Opto-RhoGEF design and activity. B) OptoTIAM (magenta) expressed in a monolayer of endothelial cells, showing cell...either CRY2 or CIB1 to your protein of interest and express the other separately, then use blue light to generate...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...The new SuperPLDs work with high efficiency in mammalian cells and in vitro, opening up a wide realm of...

  4. Let There Be LITE Plasmids

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    Blog Post
    ... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...functions, Konermann needed tools to manipulate the expression of specific genes in the brain in reversible ...demonstrated use of the LITEs to control gene expression in mouse neurons and in the brains of living ...stimulation. Under those optogenetic controls, gene expression levels reliably increased in vitro by a factor...system could be by applying it to altering gene expression via epigenetics with the addition of histone ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest... Silvana Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states...

  5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ... Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify any... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...highlight their research and how they will use plasmids and viral preps from Addgene to further their ... It’s possible that solute channels typically expressed on other cell types can compensate for or bypass...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...team will be able to validate their findings in mammalian cells with both human and murine targets thus ...defects. Eight genes from the X chromosome are expressed at significantly reduced levels in women with ...

  6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...cleavage does not occur when Cas13d is expressed in a mammalian system. Since Cas13d is functionally similar...specific editor in mammalian cells Not all Cas13d proteins are functional in mammalian cells, but Konermann...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...

  7. Plasmids 101: Biotinylation

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    Blog Post
    ...effectively biotinylate AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay...more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein ...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13 (2003)..., M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin...

  8. What's New in CRISPR - March 2020

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional...CRISPRa depended on continued expression of dCas9 and gRNAs whose expression varies depending on transgene...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...demethylation Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic...the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is inaccessible and the... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...

  9. Seeing Red: Simple GFP Photoconversion

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    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...photoconversion One concern with photoconverting GFP is the expression level necessary to observe a signal. Red signal...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells ...rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...with natural chain termination at stop codons, expression levels of these tagged proteins are often very...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...aminoacyl-tRNA Synthetase Mammalian Cells pDULE-ABK pyrrolysyl-tRNA synthase for aliphatic diazirine ...diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli ...

  11. Five Popular Model Organisms

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    Blog Post
    ...spatiotemporal expression of a gene of interest. This type of control over gene expression is possible in... (RNAi) by feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest...signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor, GCaMP6...interested in C. elegans plasmids and resources check out Addgene’s Worm Expression page. Looking for more... eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages...musculus) Let’s start with the most preferred mammalian model organism of many researchers: the mouse ...Mus musculus). Mice have many advantages as a mammalian model organism for scientists as they have a relatively...

  12. Hot Plasmids: Spring 2025

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    Blog Post
    ...special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible ...empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins, and stay...stay tuned for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...) Fluorescence from axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything...

  13. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    ... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...have previously been stably transfected for Cas9 expression. The Yusa lab has used their library to screen...possible to conduct systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one...). Genome-Wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library...

  14. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    ...pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs...for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're...packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier ...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,...

  15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency...interest. In contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...were unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

  16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ...labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of ...large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also work equally... well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral...

  17. What's New in CRISPR - December 2019

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...of bacteria that can cause disease in humans. Expression of this system is tunable via a rhamnose-inducible...compared to a mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-...lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced...packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ...Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that have been...delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery Methods”. Validating...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...modify the genome but still want to affect gene expression? dCas9 can be used as a platform to deliver various... transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...activation domains to the same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator...

  19. Sleeping Beauty Awakens for Genome Engineering

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    ...) in a plasmid backbone. A separate plasmid contains the transposase gene (red) for expressing the transposase....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...constitutive expression (pSBbi derivatives) and one set of vectors for inducible expression (pSBtet derivatives...transposase enzyme.2.) The transposase enzyme is expressed (red stars) and binds the inverted repeats (green...transposition; and 2) a transposon containing the gene-expression cassette that can translocate within the genome...many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in a new and improved...

  20. Hot Plasmids: Summer 2024

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    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...
Showing: 61 - 80 of 237 results