We narrowed to 153 results for: N-pac

...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug... survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2...

...Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine) pTP955 (3x cysteines.... Nanobodies will bind to the column due to their N-terminal His14-bdNEDD8 tag. After several washes, ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...

...Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

.... Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science..., Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature...-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol...Heidenreich M, Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

...Saunders, A., Oldenburg, I.A., Miyamichi, K., Akhtar, N., Luo, L., Whelan, S.P., Sabatini, B., and Cepko, ...K.Y., Jang, M.J., Yoo, B.B., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian...Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham, I.R., and Tonegawa S. (2014...Oh, S.W., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry,... applied, but these methods can have a negative impact on cellular health. Another limitation of traditional... them capable of diffusing in the extracellular space; they also have low immunogenicity and cellular ...less likely to be sequestered in the extracellular space. In addition, rAAV2-retro’s altered amino acid sequence...

...https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A., Kissling, L., Marquart, K. F., Schmidheini...https://doi.org/10.1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence.... L., Medema, R. H., & van Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand...

...mTFP1-MAPTau-N-10 MAPT mTFP1 CMV Parkinson's, FTD Michael Davidson 55570 mTurquoise-MAPTau-N-10 MAPT mTurquoise... mPlum-MAPTau-N-10 MAPT mPlum CMV Parkinson's, FTD Michael Davidson 56230 mIFP-MAPTau-N-10 MAPT mIFP CMV...mPA-GFP-MAPTau-N-10 MAPT GFP CMV Parkinson's, FTD Michael Davidson 57286 Dronpa-MAPTau-N-10 MAPT Dronpa....2-MAPTau-N-10 MAPT mEos3.2 CMV Parkinson's, FTD Michael Davidson 57552 mGeos2-VEL-MAPTau-N-10 MAPT mGeos2...leukoencephalopathy Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT...Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47...Parkinson's Michael J Fox Foundation MJFF 40403 pBACgus N-His/GST/LRRK2 1867-2186 LRRK2 His, TEV, GST polH Parkinson's...

...developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced...-CoV-2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...single-strand AAV (ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...proteins and a critical component for viral genome packaging. Addgene recommends both for use in western blots...that was more desirable than having many closely-packed labeled cells. Plus, Voltron2’s fluorescence is...vectors for microglia was further enhanced by packaging the virus as self-complementary AAV (scAAV). However...

...Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, ...P, Gautam A, Windsor I, Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai ... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell...

... prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J...double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

...Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta... Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...9 St. Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris...nar/gky332 Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA (2019) A ... The lab then introduced an sgRNA targeting the GFPACG to the cells. As expected, only the cells that ...luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...

...PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus... Central PMCID: PMC449739 Yew, C.T., Gurumoorthy, N., Nordin, F., Tye, G.J., Wan Kamarul Zaman, W.S., ...further! You can package your own lentiviral vector using an integrase-deficient packaging plasmid available... the integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details...integration of this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes....transcriptase), and IN (integrase). These viral packaging genes are required for the production of lentiviral...integrated into the host genome via the viral proteins packaged during production (read our blog post on the retroviral...

.../doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt, Tim Harmsen, Christ Leemans, Tao Chen, ...10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...

..., P., Ashoor, H., Åström, G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., ...Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are transcribed by RNA polymerase...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

...Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two...van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell...development of a new technique. In a structure as tightly packed and interwoven as the brain, robust single-cell...

...includes plasmids with different varieties of tags, both N- and C-terminal. Each set of plasmids uses the same...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...

...27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J. D. Sander, J. Staber, S. J. ...Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N. L. Craig, “piggyBac transposase tools for genome ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

...Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B., Errington...these milestones remind us of the extraordinary impact that happens when scientists come together to share...more AAV viral vector options, we launched AAV Packaged on Request, a service that dramatically expands... transfer plasmid in our repository can now be packaged on demand in five distinct serotypes, offering...breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank you for...

...Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson... T. M., Lander, E. S., Weissman, J. S., Friedman, N., & Regev, A. (2016). Perturb-Seq: Dissecting Molecular...Zukher, I., Dujardin, G., Sousa-Luís, R., & Proudfoot, N. J. (2023). Elongation roadblocks mediated by dCas9...vectors is that the packaging limit (how large of a DNA sequence you can actually package into your viral ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...promoters, and selection markers. There are also no packaging limits as there are for viral vectors — the only...