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We narrowed to 154 results for: N-pac

Showing: 61 - 80 of 154 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

  2. COVID-19 Resources

    Type
    Collection
    ...Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A.... small envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for ...Liu, Y.Y., Wang, Q.Y., Bian, H., Zhu, P., Chen, Z. N. (2020). CD147-spike protein is a novel route for ...neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection –...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Saunders, A., Oldenburg, I.A., Miyamichi, K., Akhtar, N., Luo, L., Whelan, S.P., Sabatini, B., and Cepko, ...K.Y., Jang, M.J., Yoo, B.B., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian...Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham, I.R., and Tonegawa S. (2014...Oh, S.W., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry,... applied, but these methods can have a negative impact on cellular health. Another limitation of traditional... them capable of diffusing in the extracellular space; they also have low immunogenicity and cellular ...less likely to be sequestered in the extracellular space. In addition, rAAV2-retro’s altered amino acid sequence...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A., Kissling, L., Marquart, K. F., Schmidheini...https://doi.org/10.1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence.... L., Medema, R. H., & van Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...Karimloo, R., Rezaiean, O., Moradzadeh, A., Mehmandoost, N., Moazzen, F., Mazraeh, A., Marmari, V., Ebrahimi,...new window) . Rao, D. D., Vorhies, J. S., Senzer, N., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities...protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors ...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced...-CoV-2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...single-strand AAV (ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...proteins and a critical component for viral genome packaging. Addgene recommends both for use in western blots...that was more desirable than having many closely-packed labeled cells. Plus, Voltron2’s fluorescence is...vectors for microglia was further enhanced by packaging the virus as self-complementary AAV (scAAV). However...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, ...P, Gautam A, Windsor I, Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai ... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J...double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

  9. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta... Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

  10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...9 St. Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris...nar/gky332 Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA (2019) A ... The lab then introduced an sgRNA targeting the GFPACG to the cells. As expected, only the cells that ...luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...

  11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus... Central PMCID: PMC449739 Yew, C.T., Gurumoorthy, N., Nordin, F., Tye, G.J., Wan Kamarul Zaman, W.S., ...further! You can package your own lentiviral vector using an integrase-deficient packaging plasmid available... the integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details...integration of this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes....transcriptase), and IN (integrase). These viral packaging genes are required for the production of lentiviral...integrated into the host genome via the viral proteins packaged during production (read our blog post on the retroviral...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013...Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman... 339(6121):823-6. PMID: 23287722 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki... P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY,...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    .../doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt, Tim Harmsen, Christ Leemans, Tao Chen, ...10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ..., P., Ashoor, H., Åström, G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., ...Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are transcribed by RNA polymerase...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  15. mTOR Pathway

    Type
    Collection
    ...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug... survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    .... Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science..., Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature...-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol...Heidenreich M, Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...mTFP1-MAPTau-N-10 MAPT mTFP1 CMV Parkinson's, FTD Michael Davidson 55570 mTurquoise-MAPTau-N-10 MAPT mTurquoise... mPlum-MAPTau-N-10 MAPT mPlum CMV Parkinson's, FTD Michael Davidson 56230 mIFP-MAPTau-N-10 MAPT mIFP CMV...mPA-GFP-MAPTau-N-10 MAPT GFP CMV Parkinson's, FTD Michael Davidson 57286 Dronpa-MAPTau-N-10 MAPT Dronpa....2-MAPTau-N-10 MAPT mEos3.2 CMV Parkinson's, FTD Michael Davidson 57552 mGeos2-VEL-MAPTau-N-10 MAPT mGeos2...leukoencephalopathy Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT...Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47...Parkinson's Michael J Fox Foundation MJFF 40403 pBACgus N-His/GST/LRRK2 1867-2186 LRRK2 His, TEV, GST polH Parkinson's...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two...van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell...development of a new technique. In a structure as tightly packed and interwoven as the brain, robust single-cell...

  19. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...includes plasmids with different varieties of tags, both N- and C-terminal. Each set of plasmids uses the same...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J. D. Sander, J. Staber, S. J. ...Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N. L. Craig, “piggyBac transposase tools for genome ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...
Showing: 61 - 80 of 154 results