We narrowed to 27 results for: N-pac

...sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia...Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of Renilla... 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko...87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral... 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko... John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal Firefly luciferase fragment for split-luciferase...NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc...

...that adds an N-terminal myristoylation signal GST Protein purification pEBG - N-terminal GST... N-terminal His6-GST-TEV for bacterial expression pDest-565 - N-terminal ...tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient expression... for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing your plasmid...c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF...PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG tag in pCS for...tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged...

... Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan, ...34478496 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L.,...Ter-Ovanesyan, D., Braff, J. L., Davidsohn, N., Housden, B. E., Perrimon, N., Weiss, R., Aach, J., Collins, J. ...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A....Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., Myhrvold, C., Bhattacharyya...Yachie, N., . . . Nureki, O. (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science...1964. PMID: 29891532 Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...

...pCMV-mGold2t-Calnexin-N-14 Endoplasmic reticulum Calnexin mGold2t Francois St-Pierre 231768 pCMV-mGold2s-Calnexin-N-14 Endoplasmic...pCMV-mGold2t-MannII-N-10 Golgi apparatus MannII mGold2t Francois St-Pierre 231769 pCMV-mGold2s-MannII-N-10 Golgi ...pCMV-mGold2t-Lysosome-N-20 Lysosomes Lamp1 mGold2t Francois St-Pierre 231770 pCMV-mGold2s-Lysosome-N-20 Lysosomes...Davidson 98822 Lck-mTurquoise2 Plasma membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella...Gadella 98821 Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057...(SKL) mCherry Maria Harrison 55146 mCherry-TOMM20-N-10 Mitochondria outer membrane TOMM20 mCherry* Michael...Cooper 189771 pLCK-mScarlet3_C1 Plasma membrane Lck N-terminal lipidation signal mScarlet3 Dorus Gadella...

... tag and an SV40 NLS at the N terminus, a 152-residue deletion from the N terminus of the wild-type TALE... The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation at rates much ...into a pCS2 expression vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA...the T7 promoter. Truncations were introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which.../mRNA synthesis vectors containing unique shorter N- and C-terminal domains (N153AA, C47AA). The reporter...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

...Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004. Specific labeling...PMID:9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell... 101131 pACP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control...backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for...

...72255 Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre... following protocol detailing the process for packaging these transfer vectors and generating new viral... WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene...

...expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93 , pAC94... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; Gateway destination...pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your input sequence...and can work for S. pyogenes , S. thermophilus , or N. meningitidis Cas9 PAMs. Currently supports: mouse...2014) (Link opens in a new window) . protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR ...destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274...

..., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. ...Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O... Kalmbach, B., Lenz, G. H., Morin, E., Nguyen, T. N., Garren, E., Bendrick, J. L., Kim, T. K., Zhou, T...Collection Caltech Systemic Capsids AAV Resources AAV Packaged on Request AAV Guide...

...Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A.... small envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for ...Liu, Y.Y., Wang, Q.Y., Bian, H., Zhu, P., Chen, Z. N. (2020). CD147-spike protein is a novel route for ...neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection –...

...Karimloo, R., Rezaiean, O., Moradzadeh, A., Mehmandoost, N., Moazzen, F., Mazraeh, A., Marmari, V., Ebrahimi,...new window) . Rao, D. D., Vorhies, J. S., Senzer, N., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities...protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors ...

... Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie Cepko 69571 pAAV-EF1a-C-CreintG...3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) minBG N/A 2 Allen Institute for Brain Science , Jonathan Ting... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases ... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

...28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013...Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman... 339(6121):823-6. PMID: 23287722 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki... P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY,...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...

...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug... survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2...

.... Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science..., Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature...-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol...Heidenreich M, Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

...mTFP1-MAPTau-N-10 MAPT mTFP1 CMV Parkinson's, FTD Michael Davidson 55570 mTurquoise-MAPTau-N-10 MAPT mTurquoise... mPlum-MAPTau-N-10 MAPT mPlum CMV Parkinson's, FTD Michael Davidson 56230 mIFP-MAPTau-N-10 MAPT mIFP CMV...mPA-GFP-MAPTau-N-10 MAPT GFP CMV Parkinson's, FTD Michael Davidson 57286 Dronpa-MAPTau-N-10 MAPT Dronpa....2-MAPTau-N-10 MAPT mEos3.2 CMV Parkinson's, FTD Michael Davidson 57552 mGeos2-VEL-MAPTau-N-10 MAPT mGeos2...leukoencephalopathy Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT...Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47...Parkinson's Michael J Fox Foundation MJFF 40403 pBACgus N-His/GST/LRRK2 1867-2186 LRRK2 His, TEV, GST polH Parkinson's...

...Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson... T. M., Lander, E. S., Weissman, J. S., Friedman, N., & Regev, A. (2016). Perturb-Seq: Dissecting Molecular...Zukher, I., Dujardin, G., Sousa-Luís, R., & Proudfoot, N. J. (2023). Elongation roadblocks mediated by dCas9...vectors is that the packaging limit (how large of a DNA sequence you can actually package into your viral ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...promoters, and selection markers. There are also no packaging limits as there are for viral vectors — the only...

...contains several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B inding D omain...Tissue Bank Antibodies (Link opens in a new window) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma...trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working with the RSRT...

...PMID: 7792603 (Link opens in a new window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C.,...15517964 (Link opens in a new window) Loew, R., Heinz, N., Hampf, M., Bujard, H., & Gossen, M. (2010). Improved...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...

...SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Park...weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) ...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function...