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We narrowed to 154 results for: N-pac

Showing: 1 - 20 of 154 results

  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...C-terminal regions of the Rep proteins. While the unique N-terminal domain of Rep78 and Rep68 contains a DNA-...experiment:  the packaging plasmid which contains the AAV structural and packaging genes, the adenoviral...on the packaging plasmid and take a look at each of its parts. Key elements of the AAV packaging plasmid...trans, freeing up space in the viral genome so that a gene of interest can be packaged into the virus instead...plasmid The packaging plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the...are required for viral genome replication and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene expression...Rep proteins from the AAV2 serotype are used to package vector genomes flanked by AAV2 ITRs into a capsid...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia...Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of Renilla... 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko...87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral... 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko... John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal Firefly luciferase fragment for split-luciferase...NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc...

  3. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The job of scientists to observe...artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of scientists and...on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years...twitter at @thompn4. New York City, NY, USA 40.7128°N 74.0059°W – Deanna McLafferty I’m a New Yorker, so...assistant and activist. Washington, DC, USA 38.9072°N 77.0369°W – Mark Lucera I am a passionate scientist...twitter at @Mark_Lucera. Ann Arbor, MI, USA 42.2808°N 83.7430°W – Alexandra Greenbaum Science and evidence-based...doesn't tweet enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist...

  4. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...10.1038/nature24644  Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, ...Silverstein, R. A., Hille, L. T., Ogul, A. N., Robinson-Garvin, N. A., Small, J. C., McCague, S., Burke, ...10.1126/science.1231143  Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...Silverstein, R. A., Kim, N., Kroell, A.-S., Walton, R. T., Delano, J., Butcher, R. M., Pacesa, M., Smith, B. K...wild-type enzyme requires a PAM sequence of NGG (where N represents any base), their engineered variants are...Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A....-y  Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A.,...s41467-023-38582-7 Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan.../10.1038/nbt.3440 Goertsen, D., Flytzanis, N. C., Goeden, N., Chuapoco, M. R., Cummins, A., Chen, Y., ...10.1038/s41593-021-00969-4 Goertsen, D., Goeden, N., Flytzanis, N. C., & Gradinaru, V. (2022). Targeting the...Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O... AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen et al., 2022 AAV.CAP-B22 CNS: neurons...Rats, marmosets, green monkeys, rhesus macaques N/A Chuapoco et al., 2023 AAV-F CNS: neurons...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G P E2A: (GSG) Q C T N Y A L L K L... L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues can...may take up precious space in viral transfer vectors with limited packaging capacity. Additionally, it may...particularly when only a portion of the plasmid is packaged for viral delivery, or the relative expression...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...10.1038/nature24644 Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, ..., Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. M. (2023). Improved...Neugebauer, M. E., Hsu, A., Arbab, M., Krasnow, N. A., McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P....10.1038/s41587-022-01532-7 Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...nature17946 Komor, A. C., Zhao, K. T., Packer, M. S., Gaudelli, N. M., Waterbury, A. L., Koblan, L. W.,...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding..., the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor et al., 2017). Gam binds the...

  8. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. MPG / AAG N-methylpurine... site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins ...mammals also have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as ...language models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and...doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N., Wei, Y., Zhou, Y., Li, Y., Wu, D., Jin, M., Cui,...10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X., Liu, N., Li, G., Wu, D., Li, Y., Jin, M., Li, H., Wei, Y....s41467-024-49343-5 Tong, H., Wang, X., Liu, Y., Liu, N., Li, Y., Luo, J., Ma, Q., Wu, D., Li, J., Xu, C.,...

  9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ..., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. ...Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O... Kalmbach, B., Lenz, G. H., Morin, E., Nguyen, T. N., Garren, E., Bendrick, J. L., Kim, T. K., Zhou, T...help propel research forward and have the biggest impact. We should know — Addgene has built its foundation...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...CIBN, where CIBN is the N-terminal fragment of CIB1 and it was fused to both the N- and C-termini of dCas9...transcription of the N-terminus is light-dependent (Yu et al., 2020). To express the N-Cas9, Haifeng Ye’s...activators p65 and VP64 which in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form ...photoreceptor, Vivid (VVD, N. crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion...transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split Cas9 nickase...split” nuclease - the authors fragmented Cas9 into N-terminal (residues 2-713, N713) and C-terminal (residues...https://doi.org/10.1093/nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Capsid Primary receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor (AAVR)2   AAV2...HGFR)3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...factor receptor (PDGFR) α and β3 AAV6 α2,3-/α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan ...D (CPD), also called AAVR25   AAV9 Terminal N-linked galactose3 AAV receptor (AAVR)2 Laminin...doi.org/10.1128/jvi.75.14.6615-6624.2001 Han, Z., Luo, N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li,...785. https://doi.org/10.3390/cells12050785 Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini...//doi.org/10.1089/hum.2019.173 Pillay, S., Meyer, N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013.../nature09523  Ibraheim R, Song C-Q, Mir A, Amrani N, Xue W, Sontheimer EJ (2018) All-in-one adeno-associated...doi.org/10.1038/ncomms14500  Levy JM, Yeh W-H, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander...doi.org/10.1038/s41551-019-0501-5  Liu J-J, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan..., Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure...08224-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo ...into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent...increase the FRET efficiency. Creating fusions at the N- and C- termini of your protein of interest The most...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein...to describe the different parts of the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 ...We have encountered several proteins for which the N- and C-terminus of the POI are necessary for its native...fluorescent protein into the POI can work well since the N- and C-terminal residues of the fluorescent protein.... Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib...detailed methods and image descriptions, a minimum n = 2, and well labeled high- and low-magnification ...Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in...Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020). Methods Matter: Standard Production Platforms...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    .../advs.202202957 Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., ...and instead introduced its opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct..., improved editing efficiencies when fused to the N terminus of a GBE construct. This effect improved ... / apyrimidinic (AP) site. UNG / UDG Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic...P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:G to G:C genome...S., Liu, L. D., Zhang, S., Zhang, Y., Wo, Y., Li, N., Wu, S., Gui, T., Wang, B., Luo, Y., Cai, Y., Liu... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...

  16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...FPs that encode N-glycosylation consensus sequences are modified with the addition of N-glycans [9]. Both...cloning strategy and decide whether you will create an N- or C-terminal fusion based on your experimental needs...were re-engineered by mutating cysteine residues and N-glycosylation consensus sequences in popular FPs. ...investigators realize too late that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions...quantitative imaging experiments or even negatively impact cells (see Figure 1). When behaving properly, ... the function of resident ER chaperones and may impact cellular function or possibly viability. FP misfolding.... It remains unclear whether the FP misfolding impacts fusion protein function and this could lead to ...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian U6 yes, cut N. meningitidis Thomson pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson...Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA1.1 64116 Mammalian/Lentiviral U6 none N. meningitidis...Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA3.1 64118 Mammalian/Lentiviral U6 none N. meningitidis...cut N. meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9/pTREX-n 68708...BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S. pyogenes... to be used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds are specific for...pyogenes Huang PX853 62885 Mammalian BbsI yes, cut, N-terminal S. pyogenes Zhang PX854 62886 Mammalian BbsI...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold...muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns have been raised over...Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...that adds an N-terminal myristoylation signal GST Protein purification pEBG - N-terminal GST... N-terminal His6-GST-TEV for bacterial expression pDest-565 - N-terminal ...tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient expression... for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing your plasmid...c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF...PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG tag in pCS for...tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged...
Showing: 1 - 20 of 154 results