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We narrowed to 114 results for: N-pac

Showing: 1 - 20 of 114 results

  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...C-terminal regions of the Rep proteins. While the unique N-terminal domain of Rep78 and Rep68 contains a DNA-...experiment:  the packaging plasmid which contains the AAV structural and packaging genes, the adenoviral...on the packaging plasmid and take a look at each of its parts. Key elements of the AAV packaging plasmid...trans, freeing up space in the viral genome so that a gene of interest can be packaged into the virus instead...plasmid The packaging plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the...are required for viral genome replication and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene expression...Rep proteins from the AAV2 serotype are used to package vector genomes flanked by AAV2 ITRs into a capsid...

  2. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The job of scientists to observe...artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of scientists and...on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years...twitter at @thompn4. New York City, NY, USA 40.7128°N 74.0059°W – Deanna McLafferty I’m a New Yorker, so...assistant and activist. Washington, DC, USA 38.9072°N 77.0369°W – Mark Lucera I am a passionate scientist...twitter at @Mark_Lucera. Ann Arbor, MI, USA 42.2808°N 83.7430°W – Alexandra Greenbaum Science and evidence-based...doesn't tweet enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist...

  3. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...10.1038/nature24644  Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, ...Silverstein, R. A., Hille, L. T., Ogul, A. N., Robinson-Garvin, N. A., Small, J. C., McCague, S., Burke, ...10.1126/science.1231143  Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...Silverstein, R. A., Kim, N., Kroell, A.-S., Walton, R. T., Delano, J., Butcher, R. M., Pacesa, M., Smith, B. K...wild-type enzyme requires a PAM sequence of NGG (where N represents any base), their engineered variants are...Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A....-y  Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A.,...s41467-023-38582-7 Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan.../10.1038/nbt.3440 Goertsen, D., Flytzanis, N. C., Goeden, N., Chuapoco, M. R., Cummins, A., Chen, Y., ...10.1038/s41593-021-00969-4 Goertsen, D., Goeden, N., Flytzanis, N. C., & Gradinaru, V. (2022). Targeting the...Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O... AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen et al., 2022 AAV.CAP-B22 CNS: neurons...Rats, marmosets, green monkeys, rhesus macaques N/A Chuapoco et al., 2023 AAV-F CNS: neurons...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G P E2A: (GSG) Q C T N Y A L L K L... L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues can...may take up precious space in viral transfer vectors with limited packaging capacity. Additionally, it may...particularly when only a portion of the plasmid is packaged for viral delivery, or the relative expression...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...10.1038/nature24644 Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, ..., Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. M. (2023). Improved...Neugebauer, M. E., Hsu, A., Arbab, M., Krasnow, N. A., McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P....10.1038/s41587-022-01532-7 Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson,...nature17946 Komor, A. C., Zhao, K. T., Packer, M. S., Gaudelli, N. M., Waterbury, A. L., Koblan, L. W.,...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding..., the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor et al., 2017). Gam binds the...

  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. MPG / AAG N-methylpurine... site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins ...mammals also have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as ...language models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and...doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N., Wei, Y., Zhou, Y., Li, Y., Wu, D., Jin, M., Cui,...10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X., Liu, N., Li, G., Wu, D., Li, Y., Jin, M., Li, H., Wei, Y....s41467-024-49343-5 Tong, H., Wang, X., Liu, Y., Liu, N., Li, Y., Luo, J., Ma, Q., Wu, D., Li, J., Xu, C.,...

  8. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ..., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. ...Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O... Kalmbach, B., Lenz, G. H., Morin, E., Nguyen, T. N., Garren, E., Bendrick, J. L., Kim, T. K., Zhou, T...help propel research forward and have the biggest impact. We should know — Addgene has built its foundation...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...CIBN, where CIBN is the N-terminal fragment of CIB1 and it was fused to both the N- and C-termini of dCas9...transcription of the N-terminus is light-dependent (Yu et al., 2020). To express the N-Cas9, Haifeng Ye’s...activators p65 and VP64 which in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form ...photoreceptor, Vivid (VVD, N. crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion...transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split Cas9 nickase...split” nuclease - the authors fragmented Cas9 into N-terminal (residues 2-713, N713) and C-terminal (residues...https://doi.org/10.1093/nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering...

  10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Capsid Primary receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor (AAVR)2   AAV2...HGFR)3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...factor receptor (PDGFR) α and β3 AAV6 α2,3-/α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan ...D (CPD), also called AAVR25   AAV9 Terminal N-linked galactose3 AAV receptor (AAVR)2 Laminin...doi.org/10.1128/jvi.75.14.6615-6624.2001 Han, Z., Luo, N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li,...785. https://doi.org/10.3390/cells12050785 Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini...//doi.org/10.1089/hum.2019.173 Pillay, S., Meyer, N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa...

  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013.../nature09523  Ibraheim R, Song C-Q, Mir A, Amrani N, Xue W, Sontheimer EJ (2018) All-in-one adeno-associated...doi.org/10.1038/ncomms14500  Levy JM, Yeh W-H, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander...doi.org/10.1038/s41551-019-0501-5  Liu J-J, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan..., Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure...08224-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo ...into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has...

  12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent...increase the FRET efficiency. Creating fusions at the N- and C- termini of your protein of interest The most...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein...to describe the different parts of the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 ...We have encountered several proteins for which the N- and C-terminus of the POI are necessary for its native...fluorescent protein into the POI can work well since the N- and C-terminal residues of the fluorescent protein.... Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib...detailed methods and image descriptions, a minimum n = 2, and well labeled high- and low-magnification ...Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in...Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020). Methods Matter: Standard Production Platforms...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    .../advs.202202957 Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., ...and instead introduced its opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct..., improved editing efficiencies when fused to the N terminus of a GBE construct. This effect improved ... / apyrimidinic (AP) site. UNG / UDG Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic...P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:G to G:C genome...S., Liu, L. D., Zhang, S., Zhang, Y., Wo, Y., Li, N., Wu, S., Gui, T., Wang, B., Luo, Y., Cai, Y., Liu... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...

  15. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...FPs that encode N-glycosylation consensus sequences are modified with the addition of N-glycans [9]. Both...cloning strategy and decide whether you will create an N- or C-terminal fusion based on your experimental needs...were re-engineered by mutating cysteine residues and N-glycosylation consensus sequences in popular FPs. ...investigators realize too late that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions...quantitative imaging experiments or even negatively impact cells (see Figure 1). When behaving properly, ... the function of resident ER chaperones and may impact cellular function or possibly viability. FP misfolding.... It remains unclear whether the FP misfolding impacts fusion protein function and this could lead to ...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold...muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns have been raised over...Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  18. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from .... I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific...recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed PMID: 2839833. PubMed Central PMCID:...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives... double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, J., Hebben, ...expression at varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids from...replication and are required for replication, packaging, and vector persistence. They work in conjunction...in the same DNA molecule as the gene that they package. In a transfer plasmid, the transgene is flanked...deleting the B-B' or C-C' arms can reduce the packaging efficiency but increase transgene expression (...Terminal Repeats (ITRs) on the Productivity, Genome Packaging, and Potency of AAV Vectors. Microorganisms, 12...Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact of Inverted Terminal Repeat Integrity on rAAV8 ...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments...2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting...Split Cas9 system The Cas9 protein is composed of an n-terminal DNA recognition domain and a mostly c-terminal...genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled out of the ...export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net...activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides users greater temporal...for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab of Tomás...
Showing: 1 - 20 of 114 results