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We narrowed to 415 results for: Numb

Showing: 61 - 80 of 415 results

  1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...particularly useful when trying to screen a limited number of cells like primary cells or in vivo. Recently...performance, an important criterion for probing a limited number of cells. Get the Brunello CRISPRko sgRNA library...essential for viability. In addition, high copy number genes may mimic viability genes as they are subject... while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto library containing only...

  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... system.  As the number of CRISPR reagents continues to grow, so too will the number of reagents available... cannot be modified using wild-type SpCas9. The number of off-target cleavage events for the VQR and VRER... and will continue to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...accessibility varied country to country.   Figure 1: Number of countries Addgene distributes to per year, 2005... 2005-2023. We identified and offered a number of solutions that increased accessibility, such as using...ice if the package stays in transit for a certain number of days and tracking average transit time for each...in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every shipping...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...plots often contain other data. Some will have numbers by gated populations; this indicates the percentage...Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage of cells in each gate... In a contour plot, each line contains the same number of events. Thus, contour lines that are close together... histograms from Alyamani et al., 2018, showing number of events within a single signal in a flow experiment...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... encoded fluorescent tag. There are, however, a number of limitations that you may run into if you choose...reflected by the pKa, which is around 6.0 for GFP. This number implies that at pH 6 only 50% of the available ...available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under acidic conditions... solutions for many of the limitations, and the number of alternatives to GFP is expected to grow with...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... from 90-160 distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful...individual neurons, the system also suffered from a number of limitations. First, the imaging of Brainbow ...expressed by a majority of neurons, limiting the number of distinct colors that could be observed in a ... of Cre-negative Brainbow mice to determine the number and type of cells in which the construct is expressed...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...with two numbers separated by a slash: the first number refers to the genome, and the second number to the...thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition, researchers...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...in your cargo. Additionally, with piggyBac™, the number of integration copies can be tightly controlled...of transposase to transposon result in a greater number of integrations per cell and a super-piggyBacTM...super-piggyBacTM can be used to get an even greater number of integrations. It should be noted, however, that piggyBac...or a dominant-negative mutant. Reducing the copy number can also mitigate the deleterious effects of expression...

  9. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...equivalent numbers of colonies on each plate (unsuccessful 3).     If you have a high number of colonies...: insert ratio” of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...successful ligation. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this by establishing...than or equal to the alpha value divided by the number of null hypotheses. Benjamini uses a complementary...largest p value where its rank divided by the total number of comparisons performed multiplied by the alpha...all sample comparison is needed, without a high number of samples (~5). Tukey’s test – Most conservative...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...individual Level 0 plasmids, or choose from a growing number of libraries containing pre-constructed standardized...utilizes a 4-module assembly system, which reduces the number of individual repeat-variable di-residue (RVD) ...hundreds of possible combinations (depending on the number of copies). This facilitates the distinction of...spatio-temporal control over expression and increases the number of species in which Brainbow may be used. This ...Max-Planck Institute of Biochemistry has developed a number of expression vectors for use with Sequence and...

  12. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...homemade competent cells will work just fine.  A number of protocols for making competent cells are available...without a heat-shock step. Because of the large number of transformations being performed at Addgene, ...your cells can be readily transformed. Compare the number of colonies obtained using your fresh batch of ...

  13. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...institutions in Europe. In the last four years alone, the number of plasmids distributed to Europe has doubled going...9,500 in 2010 to more than 20,000 in 2013. And the number of plasmids requested by scientists based in Europe... scientists in Europe. Despite these fantastic numbers, a lot of European scientists and institutions ...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ..., such as U6 or EF1α promoters, has limited the number of gRNAs that can be packaged into viral vectors...high efficiencies, which is scalable for a higher number of MPE. Therapeutic potential  To explore the therapeutic...screenings that would perturb the unprecedented number of genes with a single viral vector.   ...

  15. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...library: in other words, 100-1000x more reads than number of plasmids in the library. Analyzing the reads... the pre-amplification DNA, you’ll compare the numbers before and after. However, because there are hundreds... file from Feng Zhang’s laboratory. There are a number of other analysis scripts available, some associated...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...you’re prepping for your experiment - there are a number of outside factors that need to be considered as...thing you should do is record the manufacturer, lot number, and expiration date somewhere you can easily check...with a simple kit, and is readily available on any number of secondary antibodies. And look - if it ain’t...

  17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...sequences (NG, GAA, and GT), greatly increasing the number of genomic loci that can be targeted (Hu et al....activity, relative to eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random and...both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives to...

  18. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...Naming conventions Give every experiment a number: Numbers are a short, easy way to name files so that...know which files go together. I also prefer the number system to using dates as labels, since I often ...

  19. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...world of science publishing, and an ever increasing number of journals and publishers to choose from, it gets...needing a subscription to the journal. An increasing number of funding bodies insist that the research data...information on lab resources, such as catalogue numbers and manufacturers’ details for antibodies, plasmids...

  20. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...too costly and time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal...Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the RSF1010 origin,...particle exponentially decreases over time and the number of elapsed generations can be determined. The lab...
Showing: 61 - 80 of 415 results