We narrowed to 66 results for: OAT

1. Lab Safety for Biosafety Levels One and Two

   Type

   Protocol

   ...requires laboratory glasses in addition to coat and gloves. This lab coat should not be worn outside of the BSL...learn more about safety in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields...

2. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation...Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute purified antigen to 20 ng/µL in...concentration!). Sample Data Figure 1: The plate was coated with serial dilutions of human recombinant purified...

3. Immunocytochemistry

   Type

   Protocol

   ...PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips HeLa cells 24-well plate 4% Paraformaldehyde... 1: Seeding cells Place a sterile poly-D-lysine coated coverslip in each well of a 24-well cell culture...

4. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ...light. Caution EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this...safety goggles or a face shield, gloves and a lab coat. Analyzing Your Gel: Using the DNA ladder in the...

5. Adeno-associated virus (AAV) Guide

   Type

   Guide

   ...genome. When wild-type AAVs enter a host cell, they uncoat in the nucleus and present as linear DNA molecules...

6. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...