...glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting...proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected by... by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected by 5 GGSGGS...GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats...repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS repeats pET28CLY8...

...potential. For example, the best promoter for a given peptide can easily be tested by generating Level 1 TUs ...

.../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

...Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions. ...

...self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the split ...

...through direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for...

...Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells. She can...

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent...

...promise that we could instead have things like peptide or RNA aptamers that act as genetically encoded...

...sample of wild type cells, purified protein, or a peptide. The positive control will confirm that the procedure...

...residue of a protein or knocking out a very small peptide. In these cases, you have little flexibility as...

...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

...library of AAV9 mutants that had a small randomized peptide loop inserted in the AAV9 capsid. This mutant library...

...their broad range of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even...

...PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma...

...PMC2784028. 3. Munro, Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned...

... Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...experiments in need of reference spectra or phosphopeptide standards for quantitative assessments. The...detection of phosphorylation-dependent protein-peptide interactions (“mode #2” expression, Barber et al...Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun JW, Lou HJ, ...

...Improved light-inducible dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), ... target protein is to be sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2...allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk Blue-light...

... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...