Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 229 results for: PID

Showing: 61 - 80 of 229 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...lateral flow strips are easy to transport and work rapidly, providing reliable results in as little as an ...within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity and ...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    ...various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing...plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small...

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal background...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative...25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic of green fluorescent protein...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...their small size (Bates and Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...amine reactive dyes. DNA binding dyes, such as propidium iodide, can only enter cells with disrupted membranes...

  13. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments... is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that these...

  16. COVID-19 Resources

    Type
    Collection
    ... DETECTR to detect coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV...protease Mpro now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection...

  17. Plan Your Experiment

    Type
    Collection
    ...complex, which is delivered to cells using cationic lipids Transient expression of CRISPR components Short... J. K., Chen, Z., & Liu, D. R. (2015). Cationic lipid-mediated delivery of proteins enables efficient ...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation ...Weiss iPSCs & iPSC-NPCs Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I...Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-...

  20. Brain Initiative Collection

    Type
    Collection
    ...portfolio of biomolecular tools and emphasizes their rapid and broad dissemination to the research community...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...
Showing: 61 - 80 of 229 results