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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 228 results for: PID

Showing: 61 - 80 of 228 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... Knockout Mouse Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249...Library 117725 Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human...

  4. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative...25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic of green fluorescent protein...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...their small size (Bates and Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream...Single fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...Single fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...sensors A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...

  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...amine reactive dyes. DNA binding dyes, such as propidium iodide, can only enter cells with disrupted membranes...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments... is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that these...

  15. Antibody Plasmid Collection

    Type
    Collection
    ...single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to...

  16. COVID-19 Resources

    Type
    Collection
    ... DETECTR to detect coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV...protease Mpro now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection...

  17. Plasmids for Stem Cell Research

    Type
    Collection
    ...e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation ...Weiss iPSCs & iPSC-NPCs Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-...

  18. Plan Your Experiment

    Type
    Collection
    ...You then delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar... J. K., Chen, Z., & Liu, D. R. (2015). Cationic lipid-mediated delivery of proteins enables efficient ...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I...Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-...

  20. Brain Initiative Collection

    Type
    Collection
    ...portfolio of biomolecular tools and emphasizes their rapid and broad dissemination to the research community...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...
Showing: 61 - 80 of 228 results