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We narrowed to 1,017 results for: PLE

Showing: 61 - 80 of 1017 results

  1. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmid, including legal approval and an implementation letter DNA amplification Viral vector production...researchers with troubleshooting and results that complement published data, capturing the insights that often...

  2. p53 Pathway

    Type
    Collection
    ...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... Figure 2: Examples of simultaneous multiplex IF labeling of rat brain samples from the James Trimmer...Figure 1: Schematic of multiplex immunofluorescence approaches. A) Sequential Multiplex IF, where one target...you will need to start with a fixed sample. Depending on the sample type, this might be frozen or formalin-fixed...al, 2022 for a sample protocol.)  Simultaneous IF Depending on your needs, your sample, and targets, you..., or you can make your own. For example, to label the rat brain sample shown in Figure 2A above, Andrews... get completely washed away and leave no signal. If you see lots of extra signal in these samples where...set of samples where you label only one target in each. Compare these individually-labeled samples with ...

  5. Fluorescent Proteins: FRET

    Type
    Collection
    ...Y67C) constructs for measuring energy transfer to multiple acceptors Koushik et al. (2009) Anomalous surplus...surplus energy transfer observed with multiple FRET acceptors mTurquoise2-mVenus FRET calibration standards...

  6. Control AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...for multiplex genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets...protospacer Multiplexing CRISPR-Cas9 options before Cpf1 crRNA array Prior to this new Cpf1 multiplexing method...allow for expression of multiple gRNAs (i.e. Csy4 cleavage sequence, tRNAs, multiple individual promoters...Cpf1 counterparts. Table 2. Cas9 Multiplexing Options  Multiplexing Method Delivery Method Advantages...Xie et al  Multiplexing crRNA expression with Cpf1  The key advantage of multiplexing crRNA expression... chart below for a summary of multiplexing efficiency in vivo. Multiplex gene editing with CRISPR-Cpf1... CRISPR technologies.  It’s a simple and effective method with multiple applications (in vitro, in vivo...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple locations in the ...CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust...you add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll...Drosophila, and zebrafish!   Why use multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you...clone and transfect multiple promoter-gRNA constructs in order to target multiple sites! In this post,...we'll cover Cas9 multiplexing options, but also check out our blog post about multiplexing with Cpf1.   ...  Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct. DNA...

  9. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...scalability and efficiency of multiplex gene editing. Moreover, existing multiplex strategies have been facing...developed DAP arrays for efficient multiplex base editing (MBE) and multiplex prime editing (MPE) with only...Schematic of multiplex gene editing with DAP array   Use DAP arrays can enable efficient multiplex gene ...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target...leveraged such host-defense mechanisms to enable multiplex gene editing in a variety of cells and organisms...from a single array, has been a top choice for multiplex gene knockout or regulations, it lacks proper ...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...end up with poor droplet quality. Using a droplet generator, the sample and the droplet generation oil ...take place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures...reagents (and time)! AAV titering using droplet digital PCR Droplet digital PCR involves partitioning a PCR... approximately 20,000 droplets using water-oil emulsion technology. Each droplet contains the ingredients...emulsion containing approximately 20,000 droplets. Each droplet contains the material required for a mini...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been...negative (gray) droplets. The no template control wells should have very few positive droplets. In the image...

  11. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... cloning techniques with higher efficiency and simpler procedures have been developed. Some of these include... excessive time requirements. FastCloning is a simpler yet reliable cloning technique that was developed...insert with an additional 15-17 bases that are complementary to the overlapping vector ends. See an overview...an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates must ...

  12. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Fluorescent proteins (FPs) are also the foundation of multiple important technologies, including FRET and optogenetics...treatment can photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila... like PsMOrange and mEos add another layer of complexity to imaging, as they allow one to monitor compartment...

  13. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...dysregulation event. Diseases are often complex, with multiple layers of dysregulation from either genetic...to each cell, future applications could deliver multiple TFs to each cell, allowing for combinatorial overexpression...the specific dysregulation in the disease. For example, very few diseases manifest as a single mutation...

  14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...author “Coping with Difficult People,” identified seven types of difficult people: Hostile-aggressives, who... are a few principles that will help you resolve most conflicts, even with difficult people. If I had ...supervisor, or do you have to work with difficult people? Each time I ask this question at my workshops,...had to use one word for to summarize these principles it would be “assertiveness.” 7 strategies to become...confidently while also considering the needs of other people. Some students and postdoctoral fellows are hesitant..., especially if their supervisors are difficult people. The following seven strategies will help you to...frequent sources of conflict is miscommunication. For example, you might misunderstand your supervisor’s suggestions...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  17. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...design GGA overhang sets, particularly for high-complexity reactions? The researchers over at New England...data-driven approach might allow for an increase in the complexity. In 2018, they published a paper profiling four...2022).     Figure 1: Workflow for high complexity Golden Gate Assemblies. Image courtesy of New... three DAD tools used were key for ultra-high-complexity assembly. While the traditional rules of thumb...data-driven approach was needed to massively expand complexity. The three tools developed, outlined below, allow...traditional design rules) for successful high-complexity Golden Gate Assembly reactions. NEBridge Ligase...of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You can specify number...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... been used to date . If you have any questions, please write to us in the comments section.  FX cloning...important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS ... research. If you’d ever like to deposit a kit, please contact us at [email protected]. ...

  19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...graphic below - including some simple comparisons. The easy multiplexing afforded by PTGs will improve...knockout, or multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also... Addgene Other Techniques for gRNA Multiplexing Multiplexed and programmable regulation of gene networks...;  to observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are...editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease Csy4 from Pseudomonas... test the application of this system to gRNA multiplexing, Xie et al. constructed PTGs containing 8 gRNAs...

  20. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...see their name tag if you are one of those people. Meet people in sessions by sitting next to someone and...conferences. Conferences provide the opportunity to meet people who are interested in the same things you are on...will meet a potential collaborator Some of these people you meet will review your papers or grants in the...the future, and don’t be surprised to know that people think more favorably of scientists they know personally...depend on your national recognition and the more people you know, the larger your profile in the community...opportunities but can be overwhelming as far as meeting new people. At large conferences, look for smaller events ...media to let others know you will attend. Meet some people online to follow up with in person. Email lab members...
Showing: 61 - 80 of 1017 results